加热法人心肌钙蛋白T的提取与纯化

Method of purification and extraction of human cardiac troponin T

目的以加热法从人左心室肌组织中提取并纯化心肌肌钙蛋白Ｔ（ＣＴｎＴ）。方法取新鲜人左心室肌经捣碎、淬取、加热、透析后提取ＣＴｎＴ，以ＤＥＡＥ５２柱层析纯化。结果经ＳＤＳ－ＰＡＧＥ凝胶电泳初步鉴定，每１００ｇ左心室肌经此法可得１１．７９ｍｇＣＴｎＴ纯品，纯化后的ｃＴｎＴ为单一条带，分子量为３７ＫＤ。结论本法提取ＣＴｎＴ步骤简单，纯度尚可，可做抗原使用。

Objective To purify human cardiac troponin T from left ventricular muscle by method of heated. Methods Human heart tissue was homogenized ,heated, centrifuged .The DEAE52 cellulose column chromatography and SDS-polyacrylamide gel electrophoresis were employed for purification and identification of CTnT . Results 11. 79 mg CTnT was obtained from 100 g human cardiac left ventricular muscle.Conclusions The CTnT obtained by this method can be used as antigen.