不同产地红曲中可溶性蛋白和2种同工酶的凝胶电泳研究

Studies on Gel Electrophoresis of Soluble Protein and Two Kinds of Isodynamic Enzyme in Hongqu from Different Producing Areas

目的：考察各地红曲产生菌株间以及本实验室诱变育种所得红曲霉属真菌ＭＳ１８号菌株的亲缘关系。方法：对从各地红曲药材样品中分离纯化到的１２株红曲霉属真菌（ＭＳ０１～１２号）及ＭＳ１８号菌株进行可溶性蛋白、酯酶和超氧化物歧化酶（ＳＯＤ）的凝胶电泳分析。结果：ＭＳ０１～１２号菌株的３种电泳图谱完全一致，而ＭＳ１８号菌株的电泳图谱发生了较大变化，这与形态学研究结果是一致的。结论：ＭＳ０１～１２号菌株为同１个种，而ＭＳ１８号菌株与ＭＳ０１～１２号不是同１个种或是它们的１个变种。

Objective: To investigate the consanguinity between 12 strains of Monascus(MS 0112)separated and purified from different samples of Hongqu collected from 12 areas in China and the variant strain of Monascus(MS 18)mutated from M.purpureus. Method: Gel electrophoresis of soluble protein,esterase and superoxide dismutase(SOD)was used to study the consanguinity between strains MS 0112 and the variant strain MS 18. Results: The electrochromatophoreses of soluble protein,esterase and SOD in the 12 strains (MS 0112) are identical,but in the variant strain MS 18 are remarkably different.The results are consistent with those of morphological studies. Conclusion: Strains MS 0112 are the identical species M.purpureus,but strain MS 18 is different from strains of MS 0112,or a variant of M.purpureus.