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肝细胞内HBV DNA原位杂交的研究 被引量:1

Morphology,Distribution and Its Significance of Intrahepatic HBV DNA in Liver Disease:a Preliminary Study by in Situ Hybridization
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摘要 应用生物素标记HBV DNA(乙肝病毒脱氧核糖核酸)作探针,对129例肝病患者肝组织作原位杂交研究。HBV DNA在慢性活动性肝炎中检出率最高(80.7%),显著高于肝硬化、慢性小叶性肝炎、急性肝炎及原发性肝癌组。具有乙肝复制指标阳性患者的肝细胞内HBV DNA检出率(82.0%)明显高于非复制组(63.0%),P<0.05。本研究观察到;HBB DNA阳性肝细胞与肝细胞坏死灶关系密切,多位于肝细胞坏死灶中间或(和)周边,其中以局灶型分布的HBVDNA阳性肝细胞与肝细胞坏死灶关系密切。提示HBV复制与肝细胞坏死有关。 A biotin-labelled DNA specific for hepatitis B virus (HBV) nucleo-tide sequences was hybridized in situ to liver tissue of 129 cases with liver diseases.It was found that chronic active hepatitis (CAH) group had a significant higher prevalence (80.7%),as compared with cirrhosis,chronic lobular hepatitis (CLH),acute hepatitis and hepatocellular carcinoma (HCC) groups.HBV DNA in HBeAg positive or intrahepatic HBcAg positive patients (82.0%) was more easily detected than the single HBsAg positive and anti-HBc cases (63.0%,P<0.05).The interesting findings that expressing HBV DNA hepatocytes,particularly as focal distribution were closedly related to hepatic necrotic sites,suggest that HBV replication may occur in conjunction with hepatic necrosis.
出处 《同济医科大学学报》 CAS CSCD 北大核心 1990年第3期153-156,共4页 Acta Universitatis Medicinae Tongji
基金 "七五"期间国家重点科技攻关项目
关键词 乙肝病毒 肝细胞 DNA 原位杂交 HBV DNA in situ hybridization virus replication biotin-probe
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