磁共振评价关节腔内注射骨髓间充质干细胞治疗早期骨性关节炎

Intraarticular injection of bone marrow mesenchymal stem cells for treatment of early osteoarthritis detected by magnetic resonance

背景:作者已对FS-3D-FISP序列诊断骨性关节炎做出研究评价,该序列对早期骨性关节炎的分级与病理有较好的一致性。目的:用骨髓间充质干细胞治疗兔早期骨性关节炎,运用MR观察软骨厚度及信号的改变评价治疗效果。方法:将新西兰大白兔随机分成对照组、骨性关节炎模型组和骨髓间充质干细胞组。骨髓间充质干细胞组在造模后第4周左膝关节腔内注射骨髓间充质干细胞。分别在术后第2周,1,2及3个月行膝关节核磁共振检查,每次每组随机选3只实验兔;扫描使用FS-3D-FISP序列测量膝关节软骨厚度,T2-FSE双回波序列测量T2值治疗前后改变,并取膝关节标本行病理观察进行Mankin’s评分,评价兔膝关节骨性关节炎治疗的效果。结果与结论:与骨性关节炎组相比,骨髓间充质干细胞组MRI示关节软骨厚度接近正常,T2值减低,无关节腔积液。提示MR显示膝关节软骨厚度及信号的改变可用于评价骨性关节炎治疗前后的改变。

BACKGROUND：Authors have assessed the FS-3D-FISP sequence for diagnosis of osteoarthritis.This sequence has a good consistency of the grade and pathology of early osteoarthritis.OBJECTIVE：To observe the thickness and signal changes by magnetic resonance（MR） in the treatment of on early osteoarthritis in rabbits using bone marrow mesenchymal stem cells（BMSCs） transplantation.METHODS：New Zealand white rabbits were randomly and equally divided into a control group,osteoarthritis group and BMSCs group.Injection of BMSCs was given to the left knee joint of rabbits in the BMSCs group on week 4 after arthritis induction.MR of knee joints was done at 2 weeks,1,2 and 3 months after the injection;three rabbits were randomly selected every time from each group.Using the sequence of FS-3D-FISP,the thickness of articular cartilage was measured and the T2-FSE double-eho sequences were utilized to measure the changes of T2 value after treatment.Pathohistological changes were observed as well as Mankin scores.The effect of the osteoarthritis in rabbit was evaluated.RESULTS AND CONCLUSION：Compared with osteoarthritis group,the thickness of articular cartilage got close to normal;T2 value reduced,and without arthroedema shown in magnetic resonance imaging.Results indicated that the thickness and signal changes of the articular cartilage on MR can be used to evaluate the changes in osteoarthritis pre-and post-treatment.