甲基强的松龙联合嗅鞘细胞移植对急性脊髓损伤大鼠运动功能和诱发电位的影响

Changes in motor function and evoked potentials of rats with acute spinal cord injury following olfactory ensheathing cells transplantation combined with Methyprednisolone

背景:嗅鞘细胞移植和甲基强的松龙是两种非常有前途的治疗脊髓损伤方法,关于二者联合治疗脊髓损伤的报道较少,结果也不尽相同。目的:通过对大鼠行为学评分和诱发电位学检测了解嗅球嗅鞘细胞移植和甲基强的松龙对大鼠急性脊髓损伤的修复作用以及二者之间有无协同作用。方法:以NYU脊髓打击法建立大鼠急性T10脊髓损伤模型,术后分别注射嗅鞘细胞、甲基强的松龙、嗅鞘细胞+甲基强的松龙、无血清的DF12培养液、生理盐水。于术后8周进行后肢体感诱发电位、运动诱发电位检测,并通过BBB评分了解各组大鼠手术前、后运动功能的变化。结果与结论:术后8周,嗅鞘细胞组、甲基强的松龙组、嗅鞘细胞+甲基强的松龙组与损伤组、DF12组比较,大鼠后肢BBB评分明显升高,体感诱发电位、运动诱发电位N1波潜伏期缩短,波幅升高,差异有显著性意义(P<0.05)。嗅鞘细胞+甲基强的松龙组与嗅鞘细胞组、甲基强的松龙组比较,大鼠后肢BBB评分明显升高,体感诱发电位、运动诱发电位N1波潜伏期缩短,波幅升高,差异有显著性意义(P<0.05)。说明嗅鞘细胞移植和甲基强的松龙单独应用均可以显著促进急性脊髓损伤大鼠运动功能恢复。二者联合促进急性脊髓损伤大鼠运动功能恢复的效果更加显著。

BACKGROUND：Olfactory ensheathing cells（OECs） transplantation and Methyprednisolone（MP） are identified as two very promising methods in treating spinal cord injury（SCI）.However,there are few reports about OECs combined with MP in treating SCI,and the treatment results are different.OBJECTIVE：To investigate the behavioral score and electrophysiological effects of OECs transplantation and MP on the repair of acute spinal cord injury and whether there is a synergistic effect between OECs transplantation and MP.METHODS：Acute spinal cord injury model（T10） was established by NYU impactor.All rats were randomly divided into 6 groups：control group,SCI group,DF12 group,OECs transplantation group,MP group,and OECs ＋ MP group.Rats were injected with OECs,MP,OECs ＋ MP,DF12 serum-free medium and saline,respectively after operation.At 8 weeks following operation,motion evoked potential（MEP） and somatosensory evoked potential（SEP） tests were performed.Changes in motor function before and after operation were estimated by Basso-Beattie-Bresnahan（BBB） score.RESULTS AND CONCLUSION：Compared with the SCI and DF12 groups,BBB score was increased,the latency period in SEP and MEP was decreased and wave amplitude was increased in the OECs,MP,and OECs ＋ MP groups at 8 weeks following operation.Significant difference was detected（P 0.05）.The latency period in SEP and MEP was decreased and wave amplitude was increased in the OECs ＋ MP group compared with the OECs group and MP group,and significant difference was detected（P 0.05）.OECs transplantation and MP alone can significantly promote recovery of motor function of acute spinal cord injury rats.OECs transplantation combined with MP can more significantly promote recovery of the motor function of acute spinal cord injury rats.