兔脂肪来源干细胞在扩张皮瓣淤血模型中的应用

Application of rabbit adipose-derived stem cells to the expanded flap with congestion

背景:扩张皮瓣淤血的问题一直是整形外科的难题,以往的方法都存在应用上的局限。相关研究表明,脂肪来源干细胞可通过促进血管的增生的方法延长扩张淤血皮瓣的成活长度。目的:尝试用兔脂肪来源干细胞来解决扩张皮瓣淤血问题。方法:20只新西兰大耳白兔均制作兔耳扩张皮瓣淤血模型,10mL扩张器在耳背皮下植入,定期注水,于第20天取出扩张器,形成扩张淤血皮瓣。一侧淤血皮瓣下注射自体脂肪来源干细胞作为实验组,另一侧淤血皮瓣下注射生理盐水作为对照组。于注射后第2周观察皮瓣的成活情况,并进行病理切片观察和CD34免疫组织化学染色,定量分析血管生成情况。结果与结论:实验组皮瓣成活的长度明显长于对照组(P<0.01),病理切片显示CD34染色密度明显高于对照组(P<0.01)。提示脂肪来源干细胞可以增加血管的发生,改善皮瓣淤血情况,从而提高扩张皮瓣的成活长度。

BACKGROUND：To expand skin flap congestion is a difficulty in the plastic surgery.Previous methods are limited in application.Adipose-derived stem cells（ADSCs） can prolong the length of survived expanded skin flap.OBJECTIVE：To solve the problem of congestion in the expanded flap by rabbit ADSCs.METHODS：A total of 20 New Zealand rabbits were used to establish models of expanded flap congestion.10 mL expandor was implanted in the skin of the back of the ear.Water was regularly injected.On day 20,the expandor was obtained to form expanded congestion flap.Autologous ADSCs were injected into one side of the congestion flap as experimental group.Saline was injected into another side as control group.At 2 weeks following injection,the survival of the flap was observed.Pathological sections were observed.Immunohistochemistry staining for CD34 was performed to quantitatively analyze vascularization.RESULTS AND CONCLUSION：The survived length of flap was significantly longer in the experimental group than the control group（P 0.01）.Pathological sections demonstrated that the density of CD34 staining was significantly greater in the experimental group compared with the control group（P 0.01）.Results indicated that ADSCs can increase vascularization,improve congestion of the flap,and elevate the length in the expanded flap.