体外心脏震波治疗前后外周血内皮祖细胞数量的变化

Changes in the number of endothelial progenitor cells from peripheral blood in patients with coronary atherosclerotic heart disease before and after extracorporeal cardiac shock wave therapy

背景:体外心脏震波治疗对冠状动脉粥样硬化性心脏病患者外周血内皮祖细胞影响的机制,目前尚不清楚。目的:观察体外心脏震波治疗对冠状动脉粥样硬化性心脏病患者外周血内皮祖细胞增殖、分化的影响。方法:选择行体外心脏震波治疗冠状动脉粥样硬化性心脏病患者25例。以未行心脏震波治疗冠状动脉粥样硬化性心脏病患者10例作为对照组,从外周血获取单个核细胞,将其在EGM-2-MV培养基培养,并分析细胞形态和形成集落的数量,7d后贴壁细胞进行细胞分析和计数。用激光共聚焦显微镜鉴定分化的内皮祖细胞。结果与结论:震波治疗组患者外周血内皮祖细胞数量、形成细胞集落数较对照组明显增多(P<0.05),提示体外心脏震波治疗对冠状动脉粥样硬化性心脏病患者外周血内皮祖细胞增殖及向内皮细胞系分化有显著促进作用。

BACKGROUND：The mechanism underlying the effect of extracorporeal cardiac shock wave therapy on endothelial progenitor cells（EPCs） from peripheral blood in patients with coronary atherosclerotic heart disease（CAHD） is poorly understood.OBJECTIVE：To observe the effects of cardiac shock wave therapy on the proliferation and the differentiation of EPCs from peripheral blood in CAHD patients.METHODS：A total of 25 CAHD patients undergoing extracorporeal cardiac shock wave therapy were selected,and another 10 patients who did not receive extracorporeal cardiac shock wave therapy served as controls.Mononuclear cells obtained from peripheral blood were cultured in EGM-2-MV medium.Cell morphology and the number of colonies formed were observed and analyzed.After 7 days of culture,adherent cells were analyzed and counted.Whether EPCs differentiated or not was identified by laser confocal microscopy.RESULTS AND CONCLUSION：The number of EPCs from peripheral blood and colonies formed were significantly increased（P 0.05） compared with the control group.It is indicated that extracorporeal cardiac shock wave therapy can promote the proliferation and differentiation of EPCs from peripheral blood into endothelial cell lineage in CAHD patients.