不同质量浓度神经肽培养正常人成骨细胞的增殖活性

Proliferative activity of normal human osteoblasts cultured with neuropeptides at different concentrations

背景:大量的研究表明神经因素可调节骨代谢,迄今为止已发现5种神经肽参与骨代谢过程。目的:进一步验证神经肽类物质对培养的正常人成骨细胞主要生物学行为的影响。方法:分别以0.02,0.1,1mg/L3种质量浓度的降钙素基因相关肽、血管活性肠肽、物质P、神经肽Y、去甲肾上腺素、骨形态发生蛋白作用于成骨细胞,通过噻唑蓝法比较不同因子的效应、同一因子不同质量浓度的作用以及骨形态发生蛋白与其他5种因子复合后的效应。结果与结论:5种神经肽类物质及骨形态发生蛋白均能促进成骨细胞的增殖,其中神经肽Y效应最强。并且除了骨形态发生蛋白外,其余5种因子的效应均与剂量成反比。结果提示,5种神经肽类因子及骨形态发生蛋白均能不同程度地促进成骨细胞活性,这从一个侧面对某些肽类物质促进成骨的现象做出了解释。

BACKGROUND：A large number of studies have demonstrated that neural factors can regulate bone metabolism.To date,5 types of neuropeptides have been found to participate in bone metabolism.OBJECTIVE：To verify the effects of neuropeptides on the biological activities of normal osteoblasts in vitro.METHODS：The osteoblasts were stimulated by calcitonin gene-related peptide,substance P,vasoactive intestinal peptide,neusopeptide Y,noradrenaline,and bone morphogenetic protein（BMP） with concentrations of 0.02,0.1,1 mg/L,respectively.The effects of different factors single or associated with BMP were examined by the means of methyl thiazolyl tetrazolium.RESULTS AND CONCLUSION：The five neuropeptides and BMP could promote osteoblast proliferation.The effect of neusopeptide Y was the strongest,and the effect of other factors was negatively related with dose.Results revealed that neuropeptides and BMP could augment the activities of osteoblasts at the different extent.This could help to explain the osteoblastic improvement of neuropeptides.