病理性瘢痕中哺乳动物雷帕霉素靶蛋白/核糖体40S小亚基S6蛋白激酶的表达

Expression of mammalian target of rapamycin/P70S6K in pathologic scars

背景:近年研究表明哺乳动物雷帕霉素靶蛋白/核糖体40S小亚基S6蛋白激酶(mammalian target of rapamycin/P70S6K,mTOR/P70S6K)信号通路在某些肿瘤形成过程中具有一定作用。病理性瘢痕特别是瘢痕疙瘩具有某些肿瘤的性质,因此该信号通路在病理性瘢痕形成中可能具有重要作用。目的:观察mTOR/P70S6K信号通路在病理性瘢痕中的的作用。方法:采用免疫组织化学SP法检测磷酸化mTOR/P70S6K(p-mTOR和p-P70S6K)在瘢痕疙瘩、增生性瘢痕、非病理性瘢痕及正常皮肤组织中的表达水平。实验所取瘢痕组织来自临床上诊断明确的瘢痕患者。结果:瘢痕疙瘩和增生性瘢痕组织中p-mTOR和p-P70S6K阳性表达率高于非病理性瘢痕及正常皮肤组(P<0.05)。p-mTOR和p-P70S6K表达呈正相关(r=0.482,P<0.05)。结果提示mTOR/P70S6K信号通路的激活参与了病理性瘢痕的形成过程,且mTOR和P70S6K之间具有协同作用。

BACKGROUND：In recent years,related research has shown that mammalian target of rapamycin/P70S6K（mTOR/P70S6K） signaling pathway has a role in tumor formation.Pathologic scar,especially the keloid scar,with the nature of some tumors,therefore mTOR/P70S6K signal pathway may be of great significance in the formation of pathological scars.OBJECTIVE：To investigate the role of mTOR/P70S6K signalling pathway in pathologic scars.METHODS：SP immunohistochemical method was used to detect the expressions of phosphated mTOR（p-mTOR） and phosphated PTOS6K（p-P70S6K） p-mTOR and p-P70S6K in keloid,hypertrophic scar,non-pathologic scar and normal skin tissues.All the scar tissues were taken from the patients who were diagnosed clearly by the clinic,and they were confirmed by pathology.RESULTS AND CONCLUSION：The positive rates of p-mTOR and p-P70S6K in keloid and hypertrophic scars were higher compared with the two control groups（P 0.05）.There was a highly positive correlation between p-mTOR and p-P70S6K expression in pathologic scars（r=0.482,P 0.05）.The activation of mTOR/P70S6K signalling pathway might be involved in the formation of pathologic scar,and the factors of it may cooperate to promote the progress of pathologic scars.