人正常皮肤和瘢痕疙瘩成纤维细胞的培养及生物学差异

Cultivation and biological differences of fibroblasts derived from human normal skin and keloid

背景:成纤维细胞是创伤愈合过程中的主要效应细胞,对瘢痕疙瘩成纤维细胞进行培养可为体外研究瘢痕疙瘩提供基础。目的:比较体外培养的人正常皮肤和瘢痕疙瘩来源的成纤维细胞生物学特性的差异,为体外研究瘢痕疙瘩提供平台。方法:应用组织微粒贴壁法,对人正常皮肤和瘢痕疙瘩来源的成纤维细胞进行原代培养、传代培养、冻存及复苏,观察二者的生长增殖情况及差异。结果与结论:瘢痕疙瘩成纤维细胞的生长增殖与正常皮肤来源的成纤维细胞无明显差异,二者进入对数生长期的时间大致相同;体外培养的成纤维细胞在液氮中冻存后,细胞复苏状态良好,接种后绝大部分细胞存活,与冻存前无明显差异。说明瘢痕疙瘩来源的成纤维细胞及正常皮肤来源的成纤维细胞生长增殖无明显差异,且均可成功冻存和复苏。

BACKGROUND：Fibroblasts are the main effector cells during wound healing,thus,cultivation of fibroblasts derived from keloid provides a basis for the in vitro keloid study.OBJECTIVE：To observe the biological difference of the fibroblasts derived from normal skin and keloid.METHODS：The fibroblasts from keloid and normal skin were primary cultured,subcultured,froze,and resuscitated in vitro.The proliferation and differences of normal skin and keloid fibroblasts were observed.RESULTS AND CONCLUSION：The fibroblasts isolated from normal skin and keloid exhibited similar morphology and growth rates.The frozen cells were resuscitated successfully after cryopreservated in liquid nitrogen.There were significances of survival rate prior to and after cryopreservation.The results demonstrated that,there are no obviously differences of fibroblasts isolated from normal skin and keloid,both of which can resuscitate successfully.