活血I号对深静脉血栓形成大鼠纤溶系统功能和静脉内皮结构的影响

Effects of Huoxue No.Ⅰ decoction on fibrinolytic function and vein endothelial structure in rats with deep venous thrombosis

背景:临床应用玄参、麦冬、当归、红花等组成的活血I号方能预防人工关节置换后下肢深静脉血栓。目的:为进一步阐明活血I号作用机制,观察其对下肢深静脉血栓形成大鼠纤溶系统功能和静脉内皮结构的影响。方法:采用结扎下腔静脉的方法制备大鼠深静脉血栓模型。分别给予不同剂量的活血I号,阳性对照药为脉络舒通颗粒,模型对照组给予生理盐水,并设立不结扎静脉的假手术组。手术后不同时间点测定大鼠深静脉血栓湿质量、血浆中组织型纤溶酶原活化剂和纤溶酶原活化剂抑制物水平的变化;电镜下观察大鼠结扎后下腔静脉内皮细胞形态组织学变化情况。结果与结论:与模型组对照组相比,活血I号各组均能显著降低大鼠血栓湿质量(P<0.05或P<0.01),升高大鼠血浆组织型纤溶酶原活化剂的含量(P<0.05,P<0.01);高剂量组于造模后1,3,6d,中剂量组于造模后3,6d,低剂量组于造模后3d,均可降低大鼠血浆纤溶酶原活化剂抑制物的水平(P<0.05)。与模型对照组相比,低剂量组和高剂量组于造模第1,3天粗面内质网明显,线粒体呈凝聚态;第6天,胶原纤维开始增多。提示活血I号能有效抑制血栓的形成,保护血管内皮细胞,其作用机制与通过提高组织型纤溶酶原活化剂活性,抑制纤溶酶原活化剂的分泌及活性有关。

BACKGROUND：Huoxue No.Ⅰ decoction comprises figwort root,ophiopogonis,angelica root and safflower which can prevent deep vein thrombosis of lower limb following prosthetic replacement.OBJECTIVE：To explore the mechanism of action of Huoxue No.Ⅰ decoction by observing its effect on fibrinolytic function and vein endothelial structure in rats with deep venous thrombosis..METHODS：Animal models of deep venous thrombosis the inferior caval veins were prepared by ligation and treated by Huoxue No.Ⅰ decoction with different doses,Mailuoshutong and physiological saline,respectively.Meantime,sham-surgery group was established.The thrombus wet weight,plasma tissue-type plasminogen activator（t-PA）,plasminogen activator inhibitor（PAI） and the endotheliocyte of the inferior caval veins were detected.RESULTS AND CONCLUSION：Compared with the model control group,Huoxue No.Ⅰ decoction with different doses groups could dramatically decreased wet weight of thrombus（P 0.05 or P 0.01）,but increased serum t-PA contents（P 0.05,P 0.01）;at 1,3 and 6 days after high-dose of Huoxue No.Ⅰ decoction treatment,at 3 and 6 days after middle-dose of Huoxue No.Ⅰ decoction treatment or at 3 days after low-dose of Huoxue No.Ⅰ decoction treatment,the serum PAI levels were obviously decreased（P 0.05）.Compared with the model control group,the rough endoplasmic reticulum was obviously and mitochondria displayed condensed state at 1 and 3 days in the low-and high-dose of Huoxue No.Ⅰ decoction groups,and the collagen fiber began increasing at 6 day after operation.The findings suggest that,Huoxue No.Ⅰ decoction can inhibit deep venous thrombosis formation and protect vascular endothelial cells via increasing t-PA and inhibiting PAI activities.