聚乙烯醇-海藻酸钙组织工程复合支架的性能

Property of polyvinyl alcohol-calcium alginate as composite scaffolds for tissue engineering

背景:将不同相对分子质量及醇解度的聚乙烯醇与海藻酸钙复合,可形成具有高含水率和适宜膨胀率的组织工程支架材料,形成的多孔结构适用于组织工程支架的细胞培养。目的:采用不同成型方法分别制备聚乙烯醇-海藻酸钙复合支架材料薄膜、颗粒、海绵,探讨其作为组织工程支架材料的可行性,并找出综合性能理想的配比。方法:将不同重均分子质量和醇解度的聚乙烯醇与海藻酸钠按一定比例复合,制备聚乙烯醇-海藻酸钙复合支架。测定复合支架的含水率和膨胀率,利用扫描电镜观察样品横截面的组织形态。结果与结论:通过改变聚乙烯醇的重均分子质量、醇解度和海藻酸钠的用量,得到不同配比的复合支架,含水率在48%～93%范围,膨胀率在120%～470%范围。扫描电镜观察显示,复合材料内部组织形态结构形成多孔结构,当聚乙烯醇重均分子质量为24000,61500时,形成的孔形态结构最好。海藻酸钠用量较少时,复合支架薄膜的孔结构形态更好,以m(聚乙烯醇):m(海藻酸钠)=3:1时孔结构形态最佳;海藻酸钠用量较大时,复合支架海绵的孔结构形态更好,以m(聚乙烯醇):m(海藻酸钠)=1:4,m(聚乙烯醇):m(海藻酸钠)=1:6时最佳,材料蓬松多孔,结构规整,且孔分布均匀,满足组织工程多孔支架材料的需要。

BACKGROUND：A scaffold for tissue engineering with high water content and proper swelling ratio can be fabricated using polyvinyl alcohol （PVA） and calcium alginate （CaAlg） at different relative molecular mass and alcoholysis.The porous structure shows potential in cell culture of tissue engineering scaffolds.OBJECTIVE：To produce the thin film,particles and sponge of PVA-CaAlg composite material by different molding methods,to investigate the feasibility of serving as a scaffold for tissue engineering,and to find out the most ideal proportion according to the property.METHODS：Scaffolds of PVA-CaAlg was fabricated by blending PVA with various amounts of sodium alginate.Water content and swelling ratio of the composite scaffold were tested.Scanning electron microscopy was used to observe the configuration of the cross-section.RESULTS AND CONCLUSION：Different proportional scaffolds could be obtained with different PVA molecular weight,alcoholysis degree and different sodium alginate dosage.They exhibited high water content,between 48%-93% and swelling ratio between 120%-470%.Scanning electron microscopy proved that inner tissue of the composite material had porous structure.When the weight average molecular weight of PVA was 24 000 and 61 500,the formed porous morphology was the best.Using small amount of sodium alginate,the pore structure and morphology of composite scaffold thin film formed better,the best structure and morphology can be achieved at m （PVA）：m （sodium alginate）=3：1;using large amount of sodium alginate,the pore structure and morphology of composite scaffold sponge formed better,the best structure and morphology can be achieved at m （PVA）：m （sodium alginate）=1：4,m （PVA）：m （sodium alginate）=1：6.The material is fluffy and porous,with regular structure and uniform pore size distribution,thus meeting the requirement of tissue engineering porous scaffolds.