异体软骨细胞种植聚丙交酯乙交酯支架修复软骨缺损

Allogeneic chondrocyte transplantation into osteochondral defects using poly lactic-co-glycolic acid scaffold

背景:聚丙交酯乙交酯支架具有将免疫源性的细胞与外界环境隔离开来,减少或防止了免疫反应的作用。目的:利用异体软骨细胞复合聚丙交酯乙交酯支架构建组织工程化软骨,观察其移植兔关节缺损后的修复效果。方法:体外培养乳兔软骨细胞扩增至第3代,种植到聚丙交酯乙交酯支架,对其进行生物特性的观察和检测。新西兰长耳大白兔36只,制造软骨缺损模型,随机分成空白组、空聚丙交酯乙交酯支架移植组、软骨细胞复合聚丙交酯乙交酯支架移植组。术后12周对缺损部位进行大体、苏木精-伊红染色、免疫组织化学染色观察,并进行Wakitani组织学评分。结果与结论:兔软骨细胞体外单层培养呈多角形,细胞克隆呈铺路石状。免疫细胞化学法检测Ⅱ型胶原表达阳性。种植到聚丙交酯乙交酯支架电镜观察与支架复合良好。三组的Wakitani组织学评分分别为(13.17±0.94),(12.31±1.89),(5.96±3.47)分,组间差异具有显著性意义(P<0.05)。软骨细胞复合聚丙交酯乙交酯支架移植修复软骨缺损效果优于其他各组。提示异体软骨细胞复合聚丙交酯乙交酯支架移植可成功修复关节软骨缺损。

BACKGROUND： Poly lactic-co-glycolic acid （PLGA） scaffold can separate the immune-derived cells from the outside environment, and reduce or prevent immune response. OBJECTIVE： The purpose of this study was to evaluate the results of repairing osteochondral defects of rabbit knees with allogeneic chondrocyte transplantation with use of PLGA scaffold. METHODS： Chondrocytes isolated from cartilage of rabbits were grown in vitro until the passage III, and then were seeded on the PLGA scaffold. A full-thickness articular cartilage defect was created on the patellar groove of 36 New Zealand white rabbits. They were equally allotted to blank group, blank PLGA scaffold group, and chondrocyte-PLGA scaffold group. The defect site was examined at 12 weeks post-surgery on the basis of macroscopic appearance, hematoxylin-eosin staining, scores of Wakitani, and immunohistochemical staining. RESULTS AND CONCLUSION： Monolayer culture of rabbit chondrocytes in vitro were polygonal, cell clones were cobblestone-like. Immunocytochemical detection showed the positive expression of type Ⅱ collagen. Cells implanted to PLGA scaffold were observed a good combination. Wakitani histological scores in three groups were （13.17±0.94）, （12.31±1.89）, （5.96±3.47） points respectively, with significant differences between the groups （P0.05）. Chondrocyte transplantation using PLGA scaffold is better than the other groups for repairing osteochondral defects. Allogeneic chondrocyte-PLGA scaffold graft can successfully repair the articular cartilage defects.