SOX-9转染骨髓基质细胞与聚乳酸-羟基乙酸共聚物的生物相容性

Biocompatibility of SOX-9 transfected bone marrow stroma cells with poly lactic-co-glycolic acid complex

背景:Sox基因家族是一个新发现的基因家族,在胚胎发育、性别分化、神经系统及骨骼系统发育过程中起着重要的作用。目的:观察SOX-9基因转染骨髓基质干细胞后在聚乳酸-羟基乙酸共聚物培养生长的生物相容性,以及其复合材料修复软骨缺损动物模型的可行性。方法:将SOX-9基因转染成功后的骨髓基质细胞在聚乳酸-羟基乙酸共聚物培养生长,制备软骨缺损动物模型,将复合物植入软骨缺损,通过大体标本观察、组织学观察及Ⅱ型胶原免疫组织化学及RT-PCR检测对软骨缺损模型的修复效果。结果与结论:SOX-9基因转染骨髓基质干细胞后可以在聚乳酸-羟基乙酸共聚物上正常生长,并有Ⅱ型胶原的高表达,将复合物植入软骨缺损后,可以修复软骨缺损动物模型。聚乳酸-羟基乙酸共聚物与转染后的骨髓基质细胞具有良好的生物相容性,可以修复兔软骨缺损动物模型。

BACKGROUND：Sox gene family is a newly discovered gene family,it plays an important role in the process of embryonic development,sex differentiation,development of nervous system and skeletal system.OBJECTIVE：To investigate the biocompatibility of SOX-9 gene transfected bone marrow stroma cells （BMSCs） with poly lactic-co-glycolic acid （PLGA） complex,and the feasibility of BMSCs transfected by SOX-9 gene for repairing cartilage defect in animal models.METHODS：BMSCs successfully transfected by SOX-9 gene were cultured on PLGA copolymer,and animal models with cartilage defect were prepared.The complex was planted into defect site of animal model.The effect of repair was evaluated by macroscopic sample observation,histological observation,collagen Ⅱ immunohistochemistry and RT-PCR.RESULTS AND CONCLUSION：BMSCs transfected by SOX-9 gene grew on PLGA copolymer well and high expression of collagen Ⅱ was observed.The complex transplanted into defect site could repair the cartilage defects in animal model.There is well biocompatibility between PLGA copolymer the transfected BMSCs.The complex could repair the cartilage defect in rabbit models.