复方中药聚乳酸微球兔膝骨性关节炎关节腔注入

Intra-articular injection of polylactic acid microspheres containing compound Chinese herbal medicine into rabbits with knee osteoarthritis

背景:骨炎1号方由淫羊藿、补骨脂、当归三味中药组成,具有补肝益肾,强壮筋骨,活血化瘀,通络止痛的作用。目的:观察骨炎1号聚乳酸微球膝关节腔内注射干预骨性关节炎的可行性。方法:将新西兰大白兔以数字表法随机分成生理盐水组、玻璃酸钠组、骨炎1号聚乳酸微组。所有动物的右膝经手术制成骨性关节炎模型。造模后6周开始关节内干预注射生理盐水、玻璃酸钠、骨炎1号聚乳酸微球,1次/周,连续给药5次。最后一次给药后2周在解剖显微镜下对患膝进行大体解剖学评分,并对关节软骨进行组织形态学观察。结果与结论:与生理盐水组相比,骨炎1号聚乳酸微球组和玻璃酸钠组均可降低实验动物的关节炎指数(P<0.05)、减轻关节软骨的退变。骨炎1号聚乳酸微球组对关节的保护作用不及玻璃酸钠组,但两组间差异无显著性意义。说明骨炎1号聚乳酸微球制剂关节腔内注射干预骨性关节炎是可行的,对动物骨性关节炎具有一定的治疗作用。

BACKGROUND：Guyan Yihao is composed by epimedium,psoralen and angelica.It has effects on invigorating liver and tonifying kidney,strengthening the muscles and bones,promoting blood circulation by removing blood stasis,removing obstruction in the channels to relieve pain.OBJECTIVE：To explore the availability of intra-articular injection of Guyan Yihao microspheres on the osteoarthritis of rabbit＇s knee.METHODS：New Zealand white rabbits were divided randomly into normal saline-treated group,sodium hyaluronate-treated group,and Guyan Yihao polylactide microsphere-treated group.All of their right knees were made osteoarthritis model with Hulth method.At 6 weeks after operating,the animals were injected respectively with normal saline,sodium hyaluronate,and Guyan Yihao polylactide microspheres into the right knee once a week for 5 times.Two weeks after the last injection,the animals were sacrificed and the affected knee joints were harvested for gross anatomical and histomorphological evaluation.RESULTS AND CONCLUSION：In Guyan Yihao polylactide microsphere-treated group and sodium hyaluronate-treated group,the arthritis index was decreased significantly compared with saline-treated group （P0.05）,and articular cartilage cataplasia was relieved.There were no significant differences between Guyan Yihao polylactide microsphere and sodium hyaluronate,but Guyan Yihao was less effective than sodium hyaluronate.Guyan Yihao polylactide microspheres are available in intra-articular injection for osteoarthritis treatment.Some protective effects of Guyan Yihao on arthritis is showed in this study.