模拟构建人肝衰竭血清对体外培养肝细胞功能的影响

Effect of human hepatic failure serum on function of hepatocytes cultured in vitro:A simulation experiment

背景:生物人工肝核心材料为肝细胞,其功能好坏直接影响临床效果。目的:模拟构建一种能够和肝衰竭血清对体外培养肝细胞具有相同生物学效应的组合物。方法:观察和对比模拟肝衰竭血清、人肝衰竭血清和体积分数10%胎牛血清对培养的CL-1细胞形态学和细胞活力的变化,检测CL-1细胞酶包括乳酸脱氢酶、谷丙转氨酶、谷草转氨酶及谷胱甘肽的分泌量。结果与结论:模拟肝衰竭血清和人肝衰竭血清对培养的CL-1细胞活力,乳酸脱氢酶、谷丙转氨酶、谷草转氨酶和谷胱甘肽的分泌量的影响差异无显著性意义(P>0.05),CL-1细胞的生长均受到抑制,体积分数10%胎牛血清细胞功能良好。结果提示,模拟肝衰竭血清组合物与人肝衰竭血清对CL-1细胞的生物学效应基本相同。

BACKGROUND：The key material of bioartificial liver is the hepatocytes,whose function directly affects the clinical results.OBJECTIVE：To simulate a composite with similar biological effects with hepatic failure serum on the hepatocytes function cultured in vitro.METHODS：The efficacy of the simulated serum liver failure,human liver failure serum and 10% fetal bovine serum on the cultured CL-1 cells was observed and compared,including cell morphology and cell viability,lactate dehydrogenase,aspartate aminotransferase,alanine aminotransferase and glutathione secretion.RESULTS AND CONCLUSION：The simulation liver failure and human liver failure serum had the same efficacy on CL-1 cell viability,lactate dehydrogenase,aspartate aminotransferase,alanine aminotransferase and glutathione secretion,with no significant difference （P0.05）,CL-2 cell growth was inhibited,and CL-1 cells had good function state in 10% fetal bovine serum.The simulated liver failure serum and human liver failure serum have the same biological effect on CL-1 cells.