工艺因素对冻干骨浸泡掺锶的影响

Influence of technical factors on incorporation of strontium into freeze-dried bone

背景:冻干骨是临床上常用的自体骨替代材料,在冻干骨中掺入成骨活性元素锶将有助于冻干骨在临床上发挥更大的作用。目的:观察含锶溶液浓度和处理时间对冻干骨浸泡掺锶的影响,并对锶离子的缓释特征进行观察。方法:采用5～40mmol/L的氯化锶溶液处理冻干骨,并对其中浓度10mmol/L组分别在浸泡1.5,3,6,9d进行检测,观察在不同时间内锶元素的掺入效率;在去离子水中进行锶离子的缓释效果评价。用ICP-OES等离子体发射光谱仪对元素含量进行分析。结果与结论:随溶液浓度升高锶元素的掺入量增加,浓度在2.0%～5.1%范围。在所观察的9d时间内,锶掺入浓度随浸泡时间延长持续增加。锶元素在初期存在爆释效应,之后进入较平稳的释放阶段。30d内锶离子释放量始终高于松质骨中的钙离子释放量。提示在实验观察的范围内,采用较高含锶浓度溶液以及延长浸泡时间可以提高锶元素在冻干骨中的掺入量。掺锶冻干骨具有长期释放锶离子的能力。

BACKGROUND：Freeze-dried bone is a most commonly used bone substitute material in clinical practice.Incorporation of strontium into freeze-dried bone will promote the performance of the material in clinic.OBJECTIVE：To observe the influence of solution concentration and soaking time on incorporation of strontium into frozen dried bone by soaking method and further investigate the releasing characteriatics of strontium element from the combined material.METHODS：Strontium was incorporated into frozen dried bone with solution concentration varying from 5 to 40mmol/L.At 10mmol/L of strontium concentration,the incorporation rates at different soaking time of 1.5,3,6,9 days were analyzed.Controlled release of strontium ion was evaluated in deionized water.ICP-OES plasma emission spectroscopy was used to quantify the elements.RESULTS AND CONCLUSION：With the increase of solution concentration,incorporation of strontium into frozen dried bone increased between 2.0% and 5.1%.Within 9 days observed,incorporation of strontium also increased along with soaking time.At the initial period,there was a burst-release of strontium,then release of strontium got steady and release of strontium was always higher than calcium within 30 days.According to this study,higher concentration of strontium solution and longer soaking time can improve the quantity of strontium incorporated into frozen dried bone.Strontium-incorporated bone has the capability to release strontium ion for a long period.