藏药翁布总苷对大鼠佐剂性关节炎的治疗作用

Therapeutic effects of total glucosides of Tibetan Herb Myricaria paniculata auct non Linn. Desv on adjuvant arthritis in rats

目的:研究翁布总苷对佐剂性关节炎(AA)大鼠的治疗作用及部分机制。方法:用弗氏完全佐剂(FCA)诱导大鼠AA模型。足容积法测量继发侧足肿胀度,进行关节炎评分,测定AA大鼠血清中超氧化物歧化酶(SOD)和一氧化氮(NO)的水平,酶联免疫吸附测定法(ELISA)测定脂多糖(LPS)诱导的滑膜腔单核巨噬细胞(AM)产生IL-1、TNF-α和PGE2的水平。结果:致炎后第12天,大鼠继发性关节炎出现,同时灌胃给予不同剂量的翁布总苷及吲哚美辛,连续两周。从第24天起,翁布总苷(1.0、1.5 g/kg)对AA大鼠继发性炎症反应(继发性足肿胀、多发性关节炎)有明显的治疗作用。翁布总苷(1.0、1.5 g/kg)可不同程度纠正AA大鼠血清中低下的SOD水平,降低AA大鼠血清中NO水平,同时降低AA大鼠滑膜腔单核巨噬细胞产生过高的白介素(IL-1)、肿瘤坏死因子(TNF-α)、前列腺素E2(PEG2)。结论:翁布总苷对AA大鼠继发性炎症有治疗作用,其作用机制可能与维持细胞因子网络平衡,减少炎症介质的释放和提高机体抗氧化能力有关。

AIM：To study the therapeutic effect of total glucosides of Tibetan Herb Myricaria paniculata auct non Linn.Desv on adjuvant arthritis（AA） in rats and its mechanisms. METHODS：Freund＇s complete adjuvant（FCA） was used to induce AA in rats.The secondary paw swelling of AA rats was measured with volume meter and the polyarthritis index was scored.The changes of the levels of SOD and NO were assessed in blood serum.The synovium mononuclear macrophage（AM） productions of IL-1,TNF-α and PGE2 were estimated by enzyme linked immuno sorbent assay（ELISA）.RESULTS：The secondary inflammation of AA rats appeared on the 12th day after injection of FCA.At the same time,total glucosides of Tibetan Herb Myricaria paniculata auct non Linn.Desv and indomethacin were given to AA rats by intragastric administration for two weeks.Total glucosides of Tibetan Herb Myricaria paniculata auct non Linn.Desv（1.0,1.5 g/kg） could significantly inhibit secondary inflammatory reaction（secondary inflammatory swelling,multiple arthritis）of AA rats from the 24th day.Total glucosides of Tibetan Herb Myricaria paniculata auct non Linn.Desv could reduce the levels of the NO in blood serum and increase the activity of the SOD in blood serum.Meanwhile,total glucosides of Tibetan Herb Myricaria paniculata auct non Linn.Desv（1.0,1.5 g/kg） could remarkably down-regulate IL-1,TNF-α and PEG2 productions of AM.CONCLUSION：The results suggest that total glucosides of Tibetan Herb Myricaria paniculata auct non Linn.Desv have therapeutical effects on AA rats.Its mechanisms may be related to keeping the balance of cytokine network in AA rats and improving the capability of anti-oxidation effect.