二甲双胍对晚期糖基化终产物诱导的血管平滑肌细胞增殖的影响

Effects of metformin on the proliferation of vascular smooth muscle cells induced by advanced glycation end products

目的:观察晚期糖基化终产物(advanced glycation endproducts,AGEs)对血管平滑肌细胞(vascular smooth muscle cells,VSMCs)增殖的影响及二甲双胍的干预作用。方法:生长状态良好的VSMCs,用不同浓度的AGEs(50、100、200、400 mg/L)干预细胞不同时间(12、24、48、72 h);不同浓度的二甲双胍(10-3,10-4,5×10-5,10-5,10-6mol/L)与200 mg/L AGEs共干预不同时间(12、24、48、72 h),MTT法测定VSMCs增殖。结果:50 mg/L AGEs干预72 h时表现出促进增殖的作用,200 mg/L AGEs干预12 h即有增殖促进作用,24 h时作用最强,400 mg/L AGEs各时间点作用同200 mg/LAGEs组;10-3与10-4mol/L二甲双胍干预24 h抑制作用最强,持续到72 h,10-6mol/L抑制增殖作用降低,24 h起效,72 h时仍有作用。结论:AGEs呈浓度和时间依赖性地促进VSMCs增殖;二甲双胍可抑制AGEs诱导的VSMCs增殖作用。

AIM：To observe the effects of advanced glycation end products on the proliferation of vascular smooth muscle cells（VSMCs） and how the course are interfered with metformin. METHODS： Well-conditioned VSMCs were dealed with different concentrations of advanced glycation end products（50,100,200,400 mg/L） in different times（12,24,48,72 h）.On the basis of above treatments,different concentrations of metformin（10-3,10-4,5×10-5,10-5,10-6 mol/L） were given to interfere VSMCs,combined with the 200 mg/L AGEs on different times（12,24,48,72 h）.The proliferation of VSMCs was measured by MTT.RESULTS：50 mg/L AGEs could promote the proliferation of VSMCs at the time point of 72 h;200 mg/L AGEs began to play the role of promotion at the time point of 12 h,furthermore,24 h were the strongest.The effects of 400 mg/L AGEs was similar to those of 200 mg/L AGEs at all the time points.10-3 mol/L and 10-4 mol/L metformin had the strongest effects of inhibition at the time point of 12 h,and this effect continued to 72 h.10-6 mol/L metformin had lower inhibition on proliferation and its effective time was from 24 h to 72 h.CONCLUSION： AGEs can promote the proliferation of vascular smooth muscle cells in a concentration-and time-dependent manner;metformin can inhibit the proliferation of vascular smooth muscle cells induced by AGEs.