山茱萸环烯醚萜苷对家兔、大鼠血小板聚集及出血时间的影响

Effects of cornel iridoid glycoside on platelet aggregation and bleeding time in rabbits and rats

目的:研究山茱萸环烯醚萜苷(CIG)对血小板聚集和出血时间的影响。方法:应用比浊法测定家兔(体外实验)及血瘀模型大鼠(体内实验)的血小板聚集率;断尾法观察大鼠出血时间。采用肾上腺素加冰水刺激法制备血瘀大鼠模型。结果:在体外实验中,CIG(0.75～3 mg/mL)能明显抑制二磷酸腺苷(ADP)或花生四烯酸(AA)诱导的家兔血小板聚集(P<0.05,P<0.01),对血小板活化因子(PAF)诱导的血小板聚集无显著影响,但更大剂量(6～12 mg/mL)能抑制PAF诱导的血小板聚集(P<0.01)。在体内实验中,CIG大剂量(180 mg/kg)灌胃给药能显著抑制ADP诱导的血瘀模型大鼠血小板聚集(P<0.01),延长正常大鼠的出血时间(P<0.05)。结论:山茱萸环烯醚萜苷具有抗血小板聚集作用,大剂量能够延长出血时间。

AIM：To investigate the effects of cornel iridoid glycoside（CIG） on platelet aggregation and bleeding time. METHODS： Born＇s turbidimetry was used to measure platelet aggregation rate in rabbits（in vitro） and blood stasis model rats（in vivo）.The tail-cutting method was applied to determine the bleeding time in rats.The blood stasis rat model was established by adrenalin and ice water stimulus method.RESULTS：In vitro study showed that CIG（0.75～3 mg/mL）significantly inhibited platelet aggregation induced by ADP or AA（P0.05,P0.01）,and CIG（6～12 mg/mL）obviously inhibited platelet aggregation induced by PAF（P0.01） in rabbits.In vivo study demonstrated that intragastric administration of CIG（180 mg/kg） markedly inhibited platelet aggregation induced by ADP in blood stasis model rats（P0.01）,and prolonged the bleeding time in normal rats（P0.05）.CONCLUSION： CIG has the effect of anti-platelet aggregation,and its high dose can prolong the bleeding time.