膜联蛋白A2在食管鳞状细胞癌中的表达及其与浸润转移的关系

Annexin A2 expression in human esophageal squamous cell carcinoma and correlation with invasion and metastasis

目的 探讨膜联蛋白A2（Annexin A2）在人食管鳞状细胞癌（ESCC）中的表达变化与浸润转移的关系.方法 收集新疆医科大学第一附属医院2000年至2008年间,手术切除并经病理证实的ESCC组织作为实验组、对应癌旁5cm以上的组织作为对照组;随机选取了ESCC与癌旁配对的22对新鲜组织和175对石蜡包埋组织作为研究对象.应用实时荧光定量PCR（real-time quantitative PCR,qRT-PCR）、蛋白免疫印迹和免疫组织化学的方法,从mRNA和蛋白两个方面检测Annexin A2的表达,并分析其与临床病理参数的关系.结果 在22对新鲜ESCC与癌旁组织中,Annexin A2在癌旁组织中的mRNA表达量（0.06±0.06）显著高于癌组织（0.02±0.02,P〈0.05）;Annexin A2在癌旁组织中的蛋白表达量（0.95±0.42）高于癌组织（0.81±0.36,P〈0.05）.在175对ESCC与癌旁组织石蜡标本中,Annexin A2蛋白的阳性表达率为82.3%（144/175）,低于配对的癌旁组织中的阳性表达率[92.0%（161/175）,P〈0.05].此外,Annexin A2的表达与ESCC患者的淋巴结转移及分化程度相关（P〈0.05）,而与ESCC患者的大体类型关系不明显（P＞0.05）.结论 Annexin A2在ESCC中的低表达可能对ESCC的发生发展及浸润转移起着一定的作用.

Objective To explore Annexin A2 expression in human esophageal squamous cell carcinoma （ESCC） and investigate the correlation of Annexin A2 expression with invasion and metastasis of human ESCC. Methods From 2000 to 2008, specimens of Xinjiang medical University First Affiliated Hospital were collected. Pathologically confirmed ESCC surgical specimens were set as experimental group, and the corresponding tumor adjacent tissues located more than 5 cm far from ESCC center were set as control group. 22 fresh and 175 paraffin-embeded ESCC specimens with corresponding adjacent tissues were randomly collected as study samples. With qRT-PCR, Western-blot and immunohistochemistry, the expression of Annexin A2 were detected at the mRNA and protein level. The correlation between Annexin A2 expression and clinicopathological parameters was analyzed. Results In 22 pairs of fresh ESCC and corresponding tumor adjacent tissues, the expression of Annexin A2 at mRNA level was significantly higher in tumor adjacent tissues （0. 06 ± 0. 06） than that in ESCC （0. 02 ±0. 02） （P＜0.05 ）. Annexin A2 expression at protein level was also significantly higher in tumor adjacent tissues （0.95±0. 42） than ESCC （0.81±0. 36） （P＜0.05）. In 175 paraffin-embeded ESCC specimens and corresponding adjacent tissues, the positive rate of Annexin A2 protein expression was 82. 3% （144/175） of the ESCC samples, which was lower than corresponding tumor adjacent tissues 92. 0% （161/175）（P＜0. 05）. In addition, Annexin A2 expression was correlated with lymphoid node metastasis （P＜0.05） and pathological differentiation in patients with ESCC （P＜0.05）. However, there was no apparent correlation with gross type （P＞0. 05）. Conclusion The low expression of Annexin A2 in ESCC maybe played a potential role in the carcinogenesis, invasion and metastasis.