食管癌组织TopoⅡα和MGMT表达及其临床意义的研究

Expression and clinical significance of TopoⅡα and MGMT in esophageal carcinoma tissues

目的:探讨潮汕地区食管鳞癌组织中Ⅱ型拓扑异构酶α(TopoⅡα)和DNA修复酶MGMT的表达及其相关性和临床意义。方法:采用免疫组织化学染色方法(EnVision二步法)观察40例食管鳞癌组织、20例上切缘正常组织中TopoⅡα和MGMT的表达。结果:在正常食管黏膜上皮中TopoⅡα的阳性表达率为35%,MGMT的阳性表达率为100%;而在食管鳞癌组织中TopoⅡα的阳性表达率为80%,MGMT的阳性表达率为50%,差异均有统计学意义,P<0.001;在食管鳞癌组织中TopoⅡα、MG-MT的表达呈负相关,r=-0.976,P<0.001。结论:TopoⅡα和MGMT蛋白在食管鳞癌组织中的表达可以作为食管鳞癌早期诊断和预后判断的参考指标。

OBJECTIVE：To investigate the expression,correlation and clinical significance of TopoⅡα and MGMT in esophageal carcinoma of Chaoshan area.METHODS：Immunohistochemical method（EnVision） was used to investigate the expression of TopoⅡα and MGMT in 40 esophageal squamouscell carcinomas and 20 normal tissues of the top distance cutting edge,and analyzed its correlation and clinical significance.RESULTS：For TopoⅡα,35% expression was observed in normal esophageal tissues,but 80% expression in esophageal squamous cell carcinoma,P0.001.For MGMT,100%expression in normal tissue was observed,while 50% in esophageal squamous cell carcinoma,P0.001.The expression of TopoⅡα and MGMT in esophageal squamous cell carcinoma as a negatively correlation（r=-0.976,P0.001）.CONCLUSION：The expression of TopoⅡα and MGMT can be used as early diagnosis and prognosis biomarkers in esophageal squamous cell carcinoma tissues.