肝细胞癌组织AP-1和NF-κB蛋白表达临床意义的研究

Expression and Clinical Signficance of AP-1 and NF-κB in hepatocellular carcinoma tissues

目的:探讨转录因子激活蛋白1(AP-1)和核转录因子κB(NF-κB)在肝细胞癌(HCC)组织中的表达及其临床意义。方法:采用链菌素抗生物素-过氧化物酶法(SP)检测50例HCC组织和癌旁正常肝组织中AP-1和NF-κB的表达,并分析两者与HCC临床病理因素的关系。结果:HCC组织中AP-1(c-Jun/c-Fos)和NF-κB阳性表达率均明显高于癌旁肝组织,差异有统计学意义,P<0.01。Ac-Jun和c-Fos蛋白的表达与有无门静脉癌栓、淋巴结转移明显相关,P<0.05。NF-κB蛋白表达与病理分级明显相关,P<0.05。HCC组织中AP-1(c-Jun/c-Fos)和NF-κB表达呈正相关,r值分别为0.556和0.538,P值均<0.01。结论:AP-1、NF-κB与HCC的发生有关,且在HCC的发生发展过程中起着不同程度的作用;NF-κB可能通过上调AP-1的表达和活性而促进HCC的浸润和转移。

OBJECTIVE：To investigate the expression and clinical signficance of AP-1 and NF-κB in hepatocellular carcinoma（HCC）.METHODS：The expression of AP-1 and NF-κB protein was detected with immunohistochemical analysis in 50 cases of HCC and pericarcinoma tissues and the relationships between protein expression and clinicopathologic factors was analyzed.RESULTS：The positive expression rates of AP-1（c-Jun/c-Fos） and NF-κB protein in HCC were significantly higher than that in pericarcinoma tissues（P0.01）.The expression of AP-1（c-Jun/c-Fos）were positively related to lymph node metascasis and portal vein cancer-embolus（P0.05）.The expression of NF-κB were positively related to differentiation degree（P0.05）.The expression of NF-κB was positiye1y correlated with AP-1（c-Jun/c-Fos）（r were 0.556 and 0.538,both P0.01）.CONCLUSIONS：Expression of AP-1 and NF-κB protein may play important roles in the carcinogenesis and development of HCC and AP-1 and NF-κB may be of different roles.Through up-regulating the expression of AP-1,NF-κB accelerates the invasion and metastasis of HCC.