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核糖体展示多肽技术的原理和应用 被引量：1

Selecting and evolving peptides using ribosome display
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摘要 目的:总结国内外关于核糖体展示的原理及核糖体展示技术用于多肽筛选的研究进展。方法:应用Medline及CHKI期刊全文数据库检索系统,以"核糖体展示"和"肽"为关键词,检索1990-2009年的相关文献。纳入标准:1)核糖体展示技术的原理;2)核糖体展示技术的发展;3)核糖体展示技术用于多肽的筛选。根据纳入标准有纳入分析11篇文献。结果:核糖体展示技术将蛋白及其mRNA同时结合在核糖体上,形成mRNA-核糖体-蛋白质(多肽)三聚体,使目的蛋白的基因型和表型联系起来。该技术完全在体外进行,已成功地筛选或改造蛋白和多肽。结论:核糖体展示技术是具有应用前景的筛选工具。 OBJECTIVE：To sum up the principle and the technology of the ribosome display on research evolution of peptides screening from journal papers published both home and abroad.METHODS：＂Ribosome display＂ and ＂peptide＂ were searched as key words in papers published from 1990 to 2009 by Medline and CNKI series full-text database retrieval systems.Three criteria of selection were adopted： 1）the principle of the ribosome display technique;2）the progress of ribosome display technique;3）the application of ribosome display technique in peptides screening.Eventually 11 articles were internalized.RESULTS： To ensure the coupling of genotype and phenotype of the interest protein,ribosome display relies on non-covalent ternary complexes of mRNA,ribosome and nascent.The complexes,which are formed during in vitro translation,can directly be applied to the selection of the properties of the displayed protein and peptide.CONCLUSION： Ribosome display is about the description of the method and application to peptides.
作者 李艳 李芳
机构地区 上海市同济大学医学院附属第一妇婴保健院
出处 《中华肿瘤防治杂志》 CAS 2010年第22期1896-1898,共3页 Chinese Journal of Cancer Prevention and Treatment
基金 国家自然基金(30672232) 上海市科委(07411967)
关键词 核糖体 肽类 综述 文献 ribosome peptides review literature
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献11

  • 1Hanes J, Pluckthun A. In vitro selection and evolution of functional proteins by using ribosome display [J]. Proc Natl Acad Sci U S A, 1997, 94(10):4937-4942.
  • 2Zahnd C, Amstutz P, Pltickthun A. Ribosome display: selecting and evolving proteins in vitro that specifically bind to a target [J]. Nature Methods, 2007,4(3):269-279.
  • 3Osada E, Shimizu Y, Akbar B K, et al. Epitope mapping using ribosome display in a reconstituted cell free protein synthesis system [J]. J Biochem, 2009,145(5):693-700.
  • 4Chen S S, Yang Y M, Barankiewicz T J. Selection of IgE-bind ing aptameric green fluorescent protein (Ap-GFP) by the ribo some display (RD) platform [J]. Biochem Biophys Res Corn mun, 2008,374(3):409-414.
  • 5温浙盛,韦尉东,陈晓勤,肖勇梅,李小东,戎铁华.应用肺癌细胞株筛选抗肺癌多肽的研究[J].实用癌症杂志,2008,23(5):441-444. 被引量：2
  • 6Weichhart T, Horky M, Sollner J, et al. Functional selection of vaccine candidate peptides from Staphylococcus aureus whole-genome expression libraries in vitro [J]. Infect Immun, 2003, 71(8) :4633-4641.
  • 7Wu H Y, Zhang X L, Pan Q, et al. Functional selection of a type IV pili-binding peptide that specifically inhibits Salmonella Typhi adhesion to/ invasion of human monocytic cells [J]. Peptides, 2005,26(11) =2057-2063.
  • 8Wada A, Sawata S Y, Ito Y. Ribosome display selection of a metal-binding motif from an artificial peptide library [J]. Bio technol Bioeng, 2008,101(5): 1102-1107.
  • 9Lamla T, Erdmann V A. Searching sequence space for high-affinity binding peptides using ribosome display[J]. J Mol Biol, 2003,329(2) :381-388.
  • 10Yanagida H, Matsuura T, Yomo T. Compensatory evolution of a WW domain variant lacking the strictly conserved trp residue [J]. J Mol Evol,2008,66(1):61 71.

二级参考文献12

  • 1温浙盛,戎铁华.肿瘤细胞膜模型筛选抗肿瘤多肽新方法的建立[J].癌症,2004,23(12):1660-1665. 被引量：3
  • 2Klastersky J. Therapy of small cell lung cancer: anything new? [ J]. Eur J Cancer Clin Oncol, 1988,24(2) :107.
  • 3Wieprecht T, Apostolov O, Beyermann M, et al. Membrane binding and pore formation of the antibacterial peptide PGLa : thermodynamic and mechanistic aspects [ J ]. Biochemistry, 2000,39 (2) :442.
  • 4Epand RM. Lipid polymorphism and protein-lipid interaction [ J]. Biochim Biophys Acta, 1998,1376:353.
  • 5Machida S, Niimi S, Shi XH, et al. Design of a novel membrane-destabilizing peptide selectively acting on acidic liposones [ J ]. Biosci Biotechnol Biochem ,2000,64 ( 5 ) :985.
  • 6Maloy WL,Kari UP. Structure-Activity studies on magainins and other host defense peptides [ J]. Biopolymers, 1995,37 : 105.
  • 7Xie QH, Matsunaga S, Wen ZS, et al. In vitro system for high-throughput screening of random peptide libraries for antimicrobial peptides that recognize bacterial membranes [ J ]. Journal of Peptide Science,2006,12 (10) : 643.
  • 8Nooter K, Stoter G. Molecular mechanisms of muhidrug resistance in cancer chemotherapy[ J]. Path Res Pract, 1996, 192(7) :768.
  • 9Blondelle SE, Houghten RA. Novel antimicrobial compounds identified using synthetic combinatorial library technology [ J ].Trends Biotechnol, 1996,14 (2) :60.
  • 10Leng B, Liu XD, Chen QX. Inhibitory effects of anticancer peptide from Mercenaria on the BGC-823 cells and several enzymes [ J ]. FEBS Lett,2005,579 ( 5 ) : 1187.

共引文献1

同被引文献20

  • 1Luo D, Geng M, Noujaim AA, et al. An engineered bivalent single-chain antibody fragment that inereases antigen binding ae- tivity [J]. J Bioehem, 1997, 121 (5): 831-834.
  • 2Sepulveda J, Shoemaker CB. Design and testing of PCR primers for the eonstruction of scFv libraries representing the im- munoglobulin repertoire of rats [J]. J Immunol Methods, 2008, 332(1-2) : 92-102.
  • 3Osada E, Shimizu Y, Akbar BK, et al. Epitope mapping using ribosome display in a reconstituted cell-free protein synthesis sys- tem [J]. J Biochem, 2009, 145(5): 693-700.
  • 4Chen SS, Yang YM, Barankiewicz TJ. Selection of IgE-binding aptameric green fluorescent protein (Ap-GFP) by the ribosome display(RD) platform[J]. Biochem Biophys Res Commun, 2008, 374 (3): 409-414.
  • 5Yanagida H, Matsuura T, Yomo T. Compensatory evolution of a WW domain variant lacking the strictly conserved Trp residue [J]. J Mol Evol, 2008, 66 (1): 61-71.
  • 6Zhou L, Mao WP, Fen J, et al. Selection of scFvs speci?c for the HepG2 cell line using ribosome display [J]. J Biosci, 2009, 34(2) : 221-226.
  • 7Li F, Su PP, Lin C, et al. Ribosome display and selection of human anti-placental growth factor scFv derived from ovarian cancer patients [J]. Protein Pept Lett, 2010, 17(5): 585-590.
  • 8De Haard HJ, Van Neer N, Reurs A, et al. A large non-immu- nized human Fab fragment phage library that permits rapid isola- tion and kinetic analysis of high affinity antibodies [J]. J Biol Chem, 1999, 274(26): 18218-18230.
  • 9He M, Cooley N, Jackson A, et al. Production of human single- chain antibodies by ribosome display [J]. Methods Mol Biol, 2004, 248(5): 177-189.
  • 10Lamla T, Erdmann VA. Searching sequence space for high-affin- ity binding peptides using ribosome display [J]. J Mol Biol, 2003, 329 (2): 381-388.

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中华肿瘤防治杂志
2010年 第22期

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