沥青烟暴露工人淋巴细胞周期和某些肿瘤标志水平的观察

Effect of asphalt smoke on cycle of lymphocytes and tumor marker levels

目的:探讨沥青烟对作业工人淋巴细胞周期及肿瘤标志水平的影响。方法:选择某炭素厂沥青熔化工段职工31名作为观察组,另外选择该厂行政管理和后勤部门职工27名作为对照组,流式细胞术检测外周血淋巴细胞周期及DNA倍体,电化学发光法检测血清非小细胞癌抗原(CYFRA21-1)、神经元特异性烯醇化酶(NSE),放射免疫分析法检测癌胚抗原(CEA)、甲胎蛋白(AFP)水平。结果:观察组G0/G1、S期细胞所占比例分别为(93.37±1.53)%、(3.33±3.00)%,小于对照组(94.80±0.85)%、(3.40±1.41)%,观察组G2/M期细胞所占比例为(3.30±1.39)%,大于对照组(1.80±2.26)%,差异均无统计学意义,P>0.05。观察组细胞增殖指数(PI)、DNA相对含量(DNARC)值分别为0.07±0.02、209.93±0.50,高于对照组0.05±0.01、207.00±3.11,差异无统计学意义,P>0.05。血清CEA、NSE、AFP和CYFRA21-1水平分别为9.98±1.17、5.03±3.39、28.92±10.25和1.71±1.46,高于对照组8.55±1.51、3.24±2.00、23.85±14.93和0.84±0.50,差异无统计学意义,P>0.05。结论:观察外周血淋巴细胞各周期细胞数及血清肿瘤标志水平改变,尚不能确定沥青烟是否对作业工人外周血淋巴细胞周期及肿瘤标志水平有影响。

OBJECTIVE：To explore the change on cycle of lymphocytes and tumor marker levels of workers exposed to asphalt fume.METHODS：Thirty-one five melting asphalt workshop workers were selected as a contact group,27 workers of non-operating asphalt fume department as a control group,and the cycle of lymphocytes and tumor marker levels were examined.RESULTS： The cells of G0/G1（93.37±1.53）% and S（3.33±3.00）% in the contact group were less than those of G0/G1（94.80±0.85）% and S（3.40±1.41）% in the control group,the cells of G2/M（3.30±1.39）% in the contact group were more than those of G2/M（1.80±2.26）% in the control group,but there were no significant differencers（P0.05）.Compared with PI（0.05±0.01） and DNARC（207.00±3.11） in the control group,PI（0.07±0.02） and DNARC（209.93±0.50） in the contact group were higher,but there were no significant differences（P0.05）.The levels of CEA（9.98±1.17） and NSE（5.03±3.39） and AFP（28.92±10.25） and CYFRA21-1（1.71±1.46） in the contact group were all higher than those of CEA（8.55±1.51） and NSE（3.24±2.00） and AFP（23.85±14.93） and CYFRA21-1（0.84±0.50） in the control group,but there were no significant differences（P0.05）.CONCLUSION： There is no change on the cycle of lymphocytes and tumor marker levels of workers exposed to asphalt fume.