摘要
目的 探讨基质金属蛋白酶2（MMP-2）及其抑制因子2（TIMP-2）在子宫腺肌病（AM）在位内膜与异位内膜的表达及意义.方法 选取广州医学院附属广州市第一人民医院2006年2月至2007年9月因AM行全子宫切除术的42例病例（取其在位及异位内膜分为AM在位内膜组和AM异位内膜组）和同期因子宫肌瘤在该院行全子宫切除术的32例病例（取其内膜作为正常子宫内膜组）,应用半定量反转录聚合酶链反应（RT-PCR）检测AM在位内膜、异位内膜及正常子宫内膜中MMP-2、TIMP-2mRNA的表达.结果 （1）MMP-2 mRNA在AM异位内膜组中相对表达量（1.43±0.32）高于AM在位内膜组和正常子宫内膜组（1.22±0.35,0.97±0.37,P＜0.05）.TIMP-2 mRNA在AM异位内膜组和AM在位内膜组中均低表达,其相对表达量分别低于正常子宫内膜组（0.96±0.31,1.13±0.30,1.29±0.19,P＜0.05）.（2）MMP-2 mRNA、TIMP-2 mRNA在子宫腺肌病在位内膜组、正常子宫内膜组的表达有周期性,分泌期均高于增生期（P＜0.05）;而在AM异位内膜组中,两者分泌期与增生期则差异无统计学意义.（3）MMP-2/TIMP-2的比值在AM异位内膜组、AM在位内膜组、正常子宫内膜组中依次递减,其中AM异位内膜组与后两组差异有统计学意义（均P＜0.05）.结论 AM异位和在位内膜组织中MMP-2高表达,TIMP-2低表达,使MMP-2/TIMP-2平衡失调,异位内膜组织侵袭降解细胞外基质的能力增强.
Objective To investigate the expression and significance of matrix metalloproteinase-2 （MMP-2） and tissue inhibitor of metalloproteinase-2 （TIMP-2） in ectopic and eutopic endometrium in adenomyosis. Methods Semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） was used to determine the mRNA of MMP-2 and TIMP-2 in ectopic and eutopic endometrium from 42 patients who received panhysterectomy for adenomyosis （AM ectopic group and AM eutopic group）, and in eutopic endometrium from 32 who received panhysterectomy for uterine myoma （normal control group）, in The First Municipal People＇s Hospital of Guangzhou from February 2006 to September 2007. Results ①The relative expression level of MMP-2 mRNA in AM ectopic endometrium group （1.43±0.32） was significantly higher than that in normal group （1.22±0.35） and AM eutopic group （0.97±0.37） （P〈0.05）, whereas the relative expression level of TIMP-2 mRNA in both AM ectopic and eutopic groups （0.96±0.31 and 1.13±0.30） was significantly lower than that in the normal control group （1.29±0.19, P〈0.05）. ②The expression of MMP-2 and TIMP-2 showed phase-dependent changes in the eutopic endometrium, with higher level in secretory phase than in proliferative phase （P〈0.05）. However, in ectopic endometrium, the expression was statistically similar in both phases （P〉0.05）. ③The highest ratio of MMP-2/TIMP-2 was in ectopic endometrium, and the lowest in normal endometrium, with significant difference comparing the AM ectopic group to AM eutopic group and normal control group. Conclusion High expression of MMP-2 and low expression of TIMP-2 are found in ectopic and eutopic endometrium from AM patients. The MMP-2/TIMP-2 dysequilibrium in ectopic endometrium may underlie its enhanced activity for invasion and degradation of extracellular matrix
出处
《中华生物医学工程杂志》
CAS
2010年第6期522-525,共4页
Chinese Journal of Biomedical Engineering
基金
广东省科学技术厅基金项目（2006835930003）
