转移相关蛋白1和埃兹蛋白在膀胱尿路上皮癌中的表达

Expression of metastasis-associated protein 1 and Ezrin in bladder urothelial carcinoma

目的 检测转移相关蛋白1（MTA-1）、埃兹蛋白（Ezrin）在膀胱尿路上皮癌（BUC）组织及正常膀胱黏膜组织中的表达,探讨两者与患者临床病理及预后之间的关系.方法 采用免疫组化法检测65例手术切除的BUC组织和30例正常膀胱黏膜组织中MTA-1和Ezrin蛋白表达,分析MTA-1和Ezrin在BUC中的表达与患者临床病理参数的关系,分析MTA-1和Ezrin表达之间的相关性和对患者预后的影响.结果 65例BUC组织中MTA-1和Ezrin阳性表达率分别为72.3%（47/65）和89.2%（58/65）,30例正常膀胱黏膜组织MTA-1和Ezrin阳性表达率均为0,差异有统计学意义（均P＜0.01）.BUC组织中,MTA-1表达与临床分期、肿瘤病理分级、有无转移、是否复发有关（均P＜0.01）,Ezrin表达与临床分期、肿瘤有无转移、是否复发有关（均P＜0.01）,MTA-1与Ezrin的表达呈正相关（r=0.742,P＜0.01）.MTA-1和Ezrin联合表达组的无病生存率明显低于联合阴性表达组（P＜0.01）.结论 MTA-1和Ezrin蛋白高表达与BUC发生发展、侵袭和转移有关.

Objective To detect the expression of metastasis- associated protein 1 （MTA- 1） and Ezrin in bladder urothelial carcinoma （BUC） and normal bladder mucosa, and to investigate their correlation with clinical pathology and prognosis. Methods The expression of MTA- 1 and Ezrin proteins was detected in 65 specimens of surgically resected BUC tissue and 30 of normal bladder mucosa with immunohistochemistry. The correlation of MTA- 1 and Ezrin expressions in BUC with clinicopathological parameters, the correlation between MTA-1 and Ezrin protein expressions and their effects on prognosis were also analyzed. Results In 65 specimens of BUC tissue, the positive rates of MTA- 1 and Ezrin expression were 72.3% （n=47） and 89.2% （n=58） , respectively, in contrast to 0% for both MTA- 1 and Ezrin expressions in 30 specimens of normal bladder mucosa, which reached the level of statistical difference （all P〈0.01）. The expression of MTA- 1 in BUC tissue was closely related to the clinical stages, tumor pathological grading, metastasis and recurrence （all P〈0.01 ） , while the expression of Ezrin was closely related to the clinical stages, metastasis and recurrence （all P〈0.01 ）, and the expression of MTA-1 was positively correlated with Ezrin expression （r=0.742, P〈0.01 ）. The disease free survival （DFS） in patients with combined expression was significantly lower than that in those with negative expression of MTA- 1 and Ezrin （P〈0.01）. Conclusion The high expression of MTA-1 and Ezrin is related to the occurrence,progression, as well as invasion and metastasis of BUC.