中药热毒清对淋巴细胞膜流动性和DNA合成的影响

The Effect of Re Du Qing on the Membrane Fluidity and DNA Synthesis of Lymphocytes

本文观察了中药热毒清对小鼠脾脏淋巴细胞膜流动性及淋巴细胞DNA合成的影响,并与伴刀豆球蛋白(ConA)和植物血凝素(PHA)的作用进行比较。结果表明:热毒清使脾混合淋巴细胞或T淋巴细胞膜的流动性升高,并能拮抗ConA或PHA诱导的使膜流动性降低的效应。体外,热毒清抑制ConA转化小鼠淋巴细胞的能力,随浓度加大而增强。上述生物效应,在整体实验中并不明显。

We observed the effect of Re Du Qing,a Chinese medicine prepara-tion,on the membrane fluidity and DNA synthesis of spleen lymphocytes from Kunming mouse in comparison with the effect of ConA and PHA.The results suggested that (1) this preparation increased the membrane fluidity of either spleen mixedor T-lympnocytes,and antagonized the effect of ConA or PHA on membrane fluidity of lymphocytes,and (2) the increase of fluidity of lymphocyte membrane induced by Re Du Qing was always accompanied by the reduction of the ability of DNA synthesis of lymphocytes in vitro caused by stimulation of ConA.However,Re Du Qing (1.0 ml/each mouse),injected into tail venous,had no marked effect on the membrane fluidity of lymphocytes as compared with the saline control.The above findings indicate that Re Du Qing may be a double-direction regulator for immunoactive lymphocytes.