山葡萄成熟果皮cDNA文库的构建及ESTs初步分析

cDNA Library Construction of Vitis amurensis Berry Skins and Primary Analysis of Expressed Sequence Tags (ESTs)

以山葡萄成熟果皮为材料,采用Creator^(TM) SMART^(TM) cDNALibraryConstruction技术构建了cDNA文库。经鉴定,文库的库容量为1.001×10~6,重组率为94.1 1%,插入片段在0.25～1.9 kb。经随机测序,获得935个有效ESTs,聚类拼接后得到636条单基因簇(unigenes),包括107个重叠群(contigs)和529个单拷贝(singlets)。这些数据为今后山葡萄功能基因分离克隆和功能基因组学研究奠定了基础。

A cDNA library from the amur grape（Vitis amurensis Rupr.） was constructed by using Creator^（TM） SMART^（TM） cDNA Library Construction protocol.The library capacity was 1.001×10~6 with a recombinant rate of 94.11%and the inserted cDNA fragments ranged from 0.25 to 1.9 kb.935 available ESTs through random sequencing were obtained,and 636 unigenes which including 98 contigs and 201 singlets were generated after clustering.This study is propitious to gene cloning and functional genomic research of amur grape.