双色荧光原位杂交检测丙烯腈接触男工精子性染色体数目畸变

STUDY ON NUMERICAL CHROMOSOME ABERRATION IN HUMAN SPERM CELLS ON ACRYLONITRILE EXPOSED WORKERS USING TWO COLOR FLUORESCENCE IN SITU HYBRIDIZATION

本研究用荧光原位杂交技术（ＦＩＳＨ）对９名丙烯腈接触男工和９名健康者精子性染色体数目畸变率进行了检测，结果显示，精子性染色体杂交点荧光信号强，背景清楚，在镜下容易观察；接触组和对照组杂交效率均大于９９％。９名对照者Ｘ和Ｙ精子分别为４９１％和４９６％，Ｘ、Ｙ精子比例接近于１∶１。对照人群精子性染色体数目畸变率与其它试验室检出结果基本一致。接触组和对照组分别共计数了９１０１５和７４６７９个精子细胞核，两组精子性染色体数目畸变率分别为０．７０％和０．３５％，有显著性差异；其中接触组ＸＸ、ＹＹ、ＸＹ分别为０．１０％、０．２３％、０．３７％，明显高于对照组的０．０５％、０１０％和０２０％，表明使用荧光原位杂交技术（ＦＩＳＨ）能检出接触丙烯腈引起的精子性染色体数目畸变。

In this study, Fluorescence in situ hybridization(FISH) was used to investigate rates of sex chromosome aneuploidy in spermatozoa among 9 acrylonitrile exposed workers and 9 healthy sperm donors. 74679 sperm nuclei were counted among 9 healthy sperm donors, and 91015 sperm nuclei among 9 acrylonitrile exposed workers. The average frequency of total chromosome numerical abnormalities was 0.70% in exposure group, and 0.35% in the control. XY bearing sperm was the most common sex chromosome disomy, with an average rate of 0.37% in exposure group, and 0.20% in the control. XX and YY bearing sperm accounted for an additional 0.10% and 0.23% in exposure group, and 0.05% and 0.10% in the control. Significantly increased frequencies of total sex chromosome disomy, and XX ,YY and XY bearing sperm were observed among acrylonitrile exposed workers, as compared with healthy sperm donors. This study demonstrated that sperm FISH was a useful biomarker to detect and compare numerical chromosome aberration in human sperm cells for heritable risk assessment of acrylonitrile.