xylE-ICR转基因小鼠模型体内致突变试验的规范化研究

NORMALIZING OF xylE ICR TRANSGENIC MOUSE MODELS FOR MEASURING MUTATIONS IN VIVO

本文通过ＭＮＮＧ作用于ｘｙｌＥＩＣＲ转基因小鼠，按１／１０ＬＤ５０给药，每天１次，连续５ｄ，最后一次给药后２１ｄ，测小鼠肝组织突变率为２１．４６×１０－５，与对照组（＜４．３８×１０－５）比相差极其显著（Ｐ＜０．０１），表明该检测方法可作为规范化程序，对诱变物进行致突变研究的系统分析。

Allow MNNG administration in the xylE ICR transgenic mice. We dosed for 5 days, then count survivors 21 days after the final dose. The results showed: the spontaneous mutant frequency for xylE gene in the liver of xylE ICR transgenic mice was much lower than the MNNG induced mutant frequency. It is evident that the protocol could be used in the analysis of mutagenesis of chemicals.