摘要
本文通过MNNG作用于xylEICR转基因小鼠,按1/10LD50给药,每天1次,连续5d,最后一次给药后21d,测小鼠肝组织突变率为21.46×10-5,与对照组(<4.38×10-5)比相差极其显著(P<0.01),表明该检测方法可作为规范化程序,对诱变物进行致突变研究的系统分析。
Allow MNNG administration in the xylE ICR transgenic mice. We dosed for 5 days, then count survivors 21 days after the final dose. The results showed: the spontaneous mutant frequency for xylE gene in the liver of xylE ICR transgenic mice was much lower than the MNNG induced mutant frequency. It is evident that the protocol could be used in the analysis of mutagenesis of chemicals.
出处
《癌变.畸变.突变》
CAS
CSCD
1999年第4期172-175,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
上海市科委基金
关键词
转基因小鼠
致突变
规范化
transgenic mouse, mutagenesis, normalizing