基因重组人碱性成纤维细胞生长因子(rh-bFGF)致突变研究

STUDY ON MUTAGENICITY ON RECOMBINANT HUMAN BASIC FIBROBLAST CROWTH FACTOR

本文用Ａｍｅｓ试验，体外染色体畸变试验和微核试验对ｒｈｂＦＧＦ进行了致突变性研究。结果显示，ｒｈｂＦＧＦ的各剂量组对ＴＡ９７、ＴＡ９８、ＴＡ１００、ＴＡ１０２菌株在±Ｓ９ｍｉｘ条件下无致突变性；在±Ｓ９ｍｉｘ条件下的染色体畸变试验中，ｒｈｂＦＧＦ的４个剂量浓度对ＣＨＬ细胞染色体无畸变作用；以０．５３ｍｇ／ＫＧ·ｂｗ、２．１３ｍｇ／ＫＧ·ｂｗ、８．５０ｍｇ／ＫＧ·ｂｗ、３４．０ｍｇ／ＫＧ·ｂｗ的ｒｈｂＦＧＦ对小鼠骨髓多染红细胞无诱发微核增加作用。实验结果表明，ｒｈｂＦＧＦ无致突变作用。

This paper reported the research results of mutagenicity of rh bFGF. The results showed that rh-bFGF at 5 doses of 0.1-500μg/plate did not induce positive mutation of strains TA 97 、TA 98 、TA 100 and TA 102 with or without S 9mix in the Ames Test. The rates of CHL Chromosome aberrations of rh-bFGF at 4 doses were negative range (<5%). The rates of micronuleus assay of polychromatic erythrocytes in mice of rh-bFGF at doses of 0.53μg/KG·bw,2.13μg/KG·bw, 85μg/KG·bw,34μg/KG·bw would not be to induce increase of its micronuclei. It is proved that rh-bFGF would not be any mutagenicity in this experiment.