抗肺肿瘤转基因小鼠模型的建立

FOUNDING OF TRANSGENIC MICE MODEL FOR ANTI LUNG TUMORIGENESIS

目的建立抗肿瘤转基因小鼠模型。方法用ＢａｍＨＩ酶切表达质粒ｐＵＬＢ３２３８获取小鼠细小病毒非结构基因，通过显微注射法将其接种入小鼠受精卵内制备转基因小鼠。转基因鼠尾部组织ＤＮＡ以ＰＣＲ检测靶基因整合；整合转基因的Ｇ０Ａ６小鼠与正常Ｃ５７ＢＬ／６Ｊ小鼠配种所产Ｇ１代转基因鼠，通过ＲＴ┐ＰＣＲ检测目的基因在其肺脏等组织的转录；Ｇ１代鼠根据ＰＣＲ检测结果分整合和未整合非结构基因两组，两组均腹腔注射致肺肿瘤化学致癌剂氨基甲酸乙酯水溶液。结果Ｇ０代有４只鼠整合靶基因；Ｇ１代（Ｇ０Ａ６子代）转基因鼠的肺组织有靶基因转录；Ｇ１代整合转基因实验组小鼠被诱导肺瘤结节平均数显著少于未整合转基因的对照组（Ｐ＜０．０１）。结论抗肺肿瘤转基因小鼠模型已建立。

Objective:To produce transgenic mice model for antitumorigenesis.Methods:Found transgenic mice carrying parvovirus minute virus of mice(MVM)non structure (NS) gene by microinjecting the gene that contained the MMTV LTR promoter and MVM NS gene.Transgenic mice were identified by subjecting tail DNA to amplification by polymerase chain reaction using a set of oligonucleotides as primers.The MVM NS gene transcript was proved by RT PCR.Urethane water solution was administered by intraperitoneal injection to induce lung tumor nodes in the offspring of G 0 A6,in which the MVM NS gene is transcribed in the lung of the transgenic mice.Results:There were four mice carrying MVM NS gene in G 0 generation.The MVM NS gene was transcribed in the transgenic offspring lung of G 0A6.The average number of lung tumor nodes was significantly lower in mice group carrying MVM NS gene than MVM NS gene free group ( P <0 01) Conclusion:Anti lung tumorigenesis model had been established.