肝细胞内HBV DNA、基因产物表达及其与肝细胞坏死灶的关系

Intrahepatic HBV DNA Replication Gene Product Expressions and Their Correlation with the Foci of Hepatic Necrosis

应用原位分子杂交方法分析慢性乙肝患者肝细胞内乙型肝炎病毒脱氧核糖核酸(HBVDNA)。发现肝内HBV DNA复制部位往往与HBsAg或HBcAg表达部位相同;HBsAg与HBcAg同时在肝内表达时,数量多不均衡,提示编码HBsAg与HBcAg基因数量或复制活性不同;且观察到含HBV DNA的肝细胞数明显超过表达HBsAg、HBcAg的肝细胞数,提示胞浆HBV DNA一部分可能处于复制期,另一部分为非复制期:肝内HBV DNA复制与HBsAg、HBcAg表达对应的部位常紧紧毗邻肝细胞坏死灶,两者频率相似。

Intrahepatic HBV DNA of patients with chronic hepatitis B was detected by in situ cytohybridization assay in combination with demonstration of HBsAg and HBcAg expression,and correlated with the foci of hepatic necrosis.It was found that HBsAg and HBcAg expressing sites appeared in the same areas where HBV DNA replicated.and that when HBsAg and HBcAg simultaneously were expressedin the liver,one antigen usually dominated,the other declined.This unbalanced expression may be caused bythe gene or transcripts variance which encodes the surface or core antigen,or by the activity of each replication.The other finding is that HBV DNA-positive hepatocytes outnumbered liver cells containing HBsAg and HBcAg in a few cases.It is supposed that a part of cytoplas-mic HBV DNA was on replicative phase with gene production expression,on the contrary,another part of cytoplasmic HBV DNA may be out of replication with no gene product expression during chronic hepatitis B.Finally,HBsAg and HBcAg expressing sites corresponding to HBV DNA-positive hepatocytes were closely attached to the foci of hepatic necrosis.There was no significant difference in the frequency of HBsAg and HBcAg expression correlating to hepatic necrosis.