新生大鼠牙齿发育中的细胞凋亡

Apoptosis in the developing tooth of postnatal rat

目的 检测新生大鼠牙齿发育过程中细胞凋亡的发生并探讨其作用。方法 选择新生3天和9天的Wistar大鼠上下颌骨石蜡切片,采用末端脱氧核苷酸转移酶介导的dUTP切口末端标记法进行末端标记。结果 新生大鼠牙胚中的成釉细胞、星网状层细胞呈阳性着色,表明发生了细胞凋亡。成牙本质细胞(尤其是牙尖形成部位的成牙本质细胞)也呈阳性着色。结论 细胞凋亡是牙齿发育过程中的一个自然过程,在牙齿发育、形成中有重要作用。末端脱氧核苷酸转移酶介导的dUTP切口末端标记法是原位检测细胞凋亡的一种快速、灵敏、有效的方法。

Objective To study apoptosis in the developing tooth of postnatal rat. Methods Transferase mediated,dUTP biotin nick end labeling(TUNEL) method was utilized to label the paraffin sections of the lower and upper jaws taken from 3 days and 9 days of postnatal rats. Results Positive stains for the presence of apoptosis were observed in ameloblasts, especially in cervical loop region, and stellate reticulum cells. Odontoblasts at the tip of developing molars in which physiological osteodentin was forming were strongly positive. A few dental pulp cells were labeled. Conclusion Apotosis is a biological process, which play an important role in the formation of teeth. Apoptosis may take place in epithelial derived cells as well as mesenchymal derived cells.