日本血吸虫卵肉芽肿形成中细胞成分及其相互关系的动态观察

Dynamic Observation on Cellular Composition and Their Interrelations in Egg Granulomas in Murine Liver Infected with S. Japonicum

本文应用光镜、电镜及酶组化技术对小鼠肝内实验性日本血吸虫卵肉芽肿的形成进行了动态观察,发现各阶段中的主要细胞成分为淋巴细胞、嗜酸性白细胞和巨噬细胞。病变向晚期发展时,成纤维细胞增多。浆细胞初见于感染后4周,到晚期则集中于肉芽肿周边区。酶组化证明,最初与虫卵接触的为巨噬细胞。经电镜鉴定,见于肉芽肿内的泡沫细胞源于巨噬细胞。淋巴细胞从其超微结构看似属T细胞。这些结果显示,日本血吸虫卵肉芽肿的形成可能是以细胞免疫为主的体液和细胞免疫混合反应的结果。

The present study employed light microscopy,enzyme histochemistry and electron microscopy to observe sequentially the development of hepatic egg granulomas in NIH mice infected with S.japonicum.The results showed that the predominant cell constituents of granuloma at various stages were lymphocyte,eo-sinophils and macrophages.The fibroblasts were more abundant as the lesions became more chronic.Plasma cells appeared 4 weeks after infection and became more concentrated in the peripheral regions at the later stages.Enzyme histochemistry indicated that the cells which contacted the parasitic eggs at the earliest stages were macrophages.Electron microscopy showed the foamy cells to be macrophages in origin,Ultrastructurally lymphocytes seemed to be T cells in nature.From the above it follows that S.jaPonicum egg granuloma might be the consequence of a mixed,chiefly cellular immune reaction,and combination with humoral immune reaction