合浦珠母贝抗菌肽粗提液制备方法的优化

Optimization of the Preparation Methods for Antimicrobial Peptides from Pinctada martensii

研究了不同处理方法制备合浦珠母贝外套膜和鳃组织抗菌肽粗提液的抗菌活性,结果表明:以1.0×106cfu.mL-1无乳链球菌刺激合浦珠母贝14 h以内对其粗提液抗菌活性无显著影响;合浦珠母贝外套膜或鳃组织抗菌肽粗提液制备时,IKAT-18匀浆机的匀浆效果要优于组织匀浆器和研钵。100℃水浴处理组织粗提液10 m in可显著提高其对溶藻弧菌的抗菌活性;反复冻融则会显著降低外套膜组织粗提液的抗菌效果。

The different processing methods were performed to extract the crude antimicrobial peptides extraction from the mantle and gill tissues of Pincatda martensii,and the activities of crude extractions were compared.The result suggested that the activities of crude extractions were not changed when Pincatda martensii was induced by Streptococcus agalactiae with 1.0×106 cfu·mL-1 for 14 h;the antibacterial activity of crude extractions homogenated by IKAT-18 homogenate machine was higher than that by the tissue grinder and the agate mortar;the antibacterial activity against Vibrio alginolyticus was significantly improved when the extraction was heated at 100 ℃ for 10 min;and the antibacterial activity against V.alginolyticus was significantly reduced when the crude extractions of the mantle tissue from P.martensii was frozen and thawed repeatedly.