马钱子总碱囊泡凝胶的体外透皮扩散和抗炎作用

Experimental Study for Noisome Gel of Semen Strychni Alkaloids Extract on in vitro Transdermal Diffusion and Anti-inflammation

目的:制备马钱子总碱(SSAE)囊泡凝胶和马钱子总碱凝胶,对两者进行体外透皮扩散对比研究和抗炎作用对比研究。方法:分别制备SSAE囊泡凝胶和SSAE凝胶,用Franz扩散池对两者进行体外透皮研究,并比较两者对弗氏完全佐剂(FCA)致大鼠足跖急性肿胀的抗炎作用。结果:SSAE囊泡凝胶为浅黄色粘稠胶体;体外透皮试验表明,SSAE囊泡凝胶的累积透过量(Q)和透皮速率(J)与SSAE凝胶无明显差别。SSAE囊泡凝胶3个剂量6.25,12.5,25 mg.kg-1,SSAE凝胶25 mg.kg-1均可抑制由FCA引起的急性足跖肿胀。结论:SSAE囊泡凝胶制备工艺可行,质量稳定,检测方法可靠,药效比较试验显示SSAE囊泡凝胶起效剂量更低,抗炎作用显著,值的进一步研究。

Objective：Noisome gel and conventional gel of Semen Strychni alkaloids extract（SSAE） were prepared.The comparative studies of transdermal diffusion in vitro and anti-inflammation were performed.Method： Noisome gel and conventional gel of SSAE were prepared and the Franz-diffusion cell was employed to compare their inhibition on acute inflammatory swelling in vitro by FCA.Result： SSAE noisome gel was pale yellow viscous gel.The accumulative permeation amount（Q） and permeation rate（J） of noisome gel and conventional gel had no significant difference.Three doses of SSAE noisome gel（6.25,12.5 and 25 mg.kg-1） and high dose of SSAE gel（25 mg.kg-1） could inhibit acute inflammatory swelling by FCA.Conclusion： The results suggested that the SSAE noisome gel was valuable to be studied further.