靛玉红体外透皮研究

Indirubin of Natural Indigo in vitro Transdermal Research

目的:研究靛玉红在不同接受液和促渗剂条件下的体外透皮情况。方法:采用Franz透皮扩散池,以离体小鼠皮肤为透皮屏障,用高效液相色谱法检测不同接受液及渗透促进剂对靛玉红的累积透过量及透过率的影响。结果:不同接受液对靛玉红透过量的影响不同,其中40%乙醇生理盐水最好,透过率为28.77%;不同促渗剂对靛玉红透过量的影响不同,其中5%冰片促渗效果最好,透过率为50.23%。结论:以40%乙醇生理盐水为接受液,冰片为促渗剂,靛玉红的体外渗透效果最好。

Objective：To study the condition of agent in vitro transdermal of indirubin under different accepted liquid and penetration enhancers.Method： The influence of indirubin accumulated permeability and through percentage of indirubin on different acceptable solution and penetration enhancers was detected by HPLC and Franz’s diffusion cell with excised mouse skin as percutaneous permeation barrier.Result： The effect of permeability of indirubin with different accepted liquid was different,the best accepted liquid was 40% ethanol saline and the through percentage was 28.77%;the effect of permeability of indirubin with different penetration enhancers was different,the best penetration enhancer was 5% borneol and the through percentage was 50.23%.Conclusion： The best effect of permeability of indirubin was with the condition of 40% ethanol saline as the accepted liquid and 5% borneol as the penetration enhancers.