制首乌辅料黑豆的质量控制研究

Study on Adjuvant Black Beans of Radix Polygoni Multiflori Preparata Quality Control

目的:建立制首乌辅料黑豆原料及其在炮制品中的质量控制方法。方法:通过比较黑豆、豆制何首乌和清蒸何首乌HPLC色谱图差异,确定制何首乌辅料黑豆的质量控制指标,在此基础上,建立豆制何首乌中黑豆的检测方法和黑豆原料的质量控制方法。结果:通过HPLC色谱图比较,检测到豆制何首乌中的微量大豆苷元。建立了制何首乌中黑豆的TLC色谱检测方法。采用HPLC,建立了黑豆原料中大豆苷元含量测定方法,测定了12批不同产地黑豆,以广东产黑豆大豆苷元含量最高。结论:本方法可作为制首乌辅料黑豆原料及其在炮制品中的质量控制方法。

Objective：To establish the quality control methods of adjuvant black beans and that in Radix Polygoni Multiflori preparata.Method： By comparing differences of HPLC chromatogram of black beans.Radix Polygoni Multiflori preparata without adjuvant and processing with black beans,to determine quality control index of adjuvant black beans.On this basis,to establish the detection method of black beans in Radix Polygoni Multiflori preparata and the quality control method of raw black beans.Result：Comparison by HPLC chromatogram,detected in trace daidzein in Radix Polygoni multiflori preparata with adjuvant,and then established the TLC Chromatography of black bean in Radix Polygoni Multiflori preparata.On the other hand,HPLC determination method of assaying daidzein in raw black beans has been established.Black beans from 12 different areas were determined,that from Guangdong province was the highest content of all.Conclusion： This method could be used as the quality control methods of adjuvant black beans and that in Radix Polygoni Multiflori Preparata.