白花丹参不同部位的红外光谱三级鉴定

Study on Identification of Different Parts of Salvia miltiorrhiza by Multi-steps Infrared Macro-fingerprint Methed

目的:对白花丹参不同部位进行红外光谱分析。方法:采用红外光谱三级鉴定法(红外光谱、二阶导数谱以及二维相关谱),对白花丹参根、茎、叶、花的粉末、脂溶性和水溶性提取物进行分析鉴定。结果:叶在有效成分特征吸收区1 800～1 200 cm-1波段峰强高于根、茎、花。一维图谱中,除花外,脂溶性提取物根、茎、叶均有邻醌C=O特征吸收1 692,1 670 cm-1峰出现;水溶性提取物叶、花的丹酚酸B芳香化合物骨架振动1 610 cm-1峰较根、茎明显。二阶导数谱中,脂溶性提取物和水溶性提取物均出现标准品谱图的特征峰,印证一维谱图结论;二维相关光谱中,白花丹参不同部位自动峰位置及强度均不相同。结论:白花丹参不同部位化学成分种类及含量有所差异,茎、叶、花是值得进一步开发利用的资源。

Objective：The different parts of Salvia miltiorrhiza Bge f.alba was analysed by IR spectroscopy.Method： Multi-steps infrared macro-fingerprint methed（infrared spectroscopy,secondary derivative spectroscopy and two dimensional correlation infrared spectroscopy） was used to analyse the powder,ethanol-soluble and water-soluble extracts of the root,stem,leaf,flower of S.miltiorrhiza.Result： In effective component characteristics absorption area（1 800-1 200 cm-1 band） the peak intensity of the leaf was higher than root,stem and flower.In the one-dimensional spectra,except flower,the ethanol soluble extract of root,stem and leaf all appeared the adjacent quinone C = O characteristic peak absorption 1 692 cm-1 or 1 670 cm-1.In water-soluble extract,the skeleton vibration peak 1 610 cm-1 of aromatic compounds（like salvianolic acids B） in leaf and flower was more obvious than root and stem.In the secondary derivative spectra,ethanol-soluble extract and water-soluble extracts all appeared the characteristic peak of standard substance,which confirm the conclusions of one-dimensional spectrograms.In the two-dimensional correlation spectra,the automatic peak position and intensity of different parts of S.miltiorrhiza were different.Conclusion： The kinds and content of chemical composition of different part of S.miltiorrhiza were different,and the stem,the leaf and the flower were resources to be further exploited.