知母与盐知母对甲亢阴虚大鼠红细胞膜Na^+-K^+-ATP酶影响的比较研究

Influences of Anemarrhenae Rhizoma and One Processed with Salt-Water on Na^+-K^+-ATP Enzyme in Erythrocyte Membrane in Rats with Hyperthyroidism and Yin Deficiency

目的:比较知母及盐知母对甲亢阴虚大鼠红细胞膜Na+-K+-ATP酶活力的影响,从能量代谢方面探讨知母盐炙后增强滋阴降火作用的炮制原理。方法:SD大鼠,雌雄各半,随机分为空白组,模型组,六味地黄丸组(2.2 g.kg-1),生知母、盐知母高剂量组(2 g.kg-1),生知母、盐知母低剂量组(1 g.kg-1);各组大鼠每天上午ig甲状腺素液2 mL(1.25 g.L-1),空白组ig生理盐水;下午各给药组ig相应的药液(10 mL.kg-1),空白组、模型组ig等体积生理盐水,3周后测定红细胞膜Na+-K+-ATP酶活性。结果:与模型组相比,生知母及盐知母各剂量组均能显著降低Na+-K+-ATP酶的活性(P<0.05),其中低剂量组盐知母作用优于生知母(P>0.05);高剂量组结果相反,即盐炙后作用降低(P>0.05),且高剂量组作用弱于低剂量组(P<0.05)。结论:知母及盐知母均具有滋肾阴清虚热的作用。

Objective：To compare the influences of Anemarrhenae Rhizoma and the one processed with salt-water on erythrocyte membrane Na ＋-K ＋-ATP enzyme activities in rats with hyperthyroidism and yin deficiency,and to clarify the nourishing yin principle by processed Anemarrhenae Rhizoma with salt water.Method： SD rats,half male and half female,were randomly divided into control group,model group,Liuwei Dihuang Wan group（2.2 g.kg-1）,high dose Anemarrhenae Rhizoma group and the high dose group of Anemarrhenae Rhizoma processed with salt-water（2 g.kg-1）,low dose Anemarrhenae Rhizoma group and accordingly the one processed with salt-water group（1 g.kg-1）.Each rat was ig given thyroxine liquid 2 mL（1.25 g.L-1） every morning.Control group was ig given physiological saline.The treatment groups were ig given corresponding liquids（10 mL.kg-1）each afternoon.Control group and model group was ig given the same volume of saline.Continuous treatment for three weeks and then the activity of erythrocyte membrane Na ＋-K ＋-ATP enzyme was determined.Result： Anemarrhenae Rhizoma and the one processed with salt-water could significantly reduce Na ＋-K ＋-ATP enzyme activity（P 0.05）.In the low dose group,the effect for the one processed with salt-water was better,but the effect of the high dose groups appeared to be on the contrary.Conclusion： Anemarrhenae Rhizoma and the one processed with salt-water both can nourish kidney yin and clear asthenic fever.