复智胶囊对血管性痴呆大鼠脑组织海马区诱导型一氧化氮合酶表达的影响

Influence of Fuzhi Capsule on Expression of Inducible Nitric Oxide Synthase in Hippocampus in Rats with Vascular Dementia

目的:观察复智胶囊对血管性痴呆大鼠的作用及脑组织海马区诱导型一氧化氮合酶表达的影响,探讨复智胶囊治疗血管性痴呆的疗效和机制。方法:60只SPF大鼠随机分为4组,每组15只,采用双侧颈总动脉结扎的方法制备血管性痴呆大鼠模型,然后分别为复智胶囊组ig复智胶囊液3.0 g.kg-1,安理申组ig安理申液0.05 mg.g-1,假手术组和模型组均ig生理盐水10 mL.kg-1,均1次/d,连续给药4周后进行行为学分析和脑组织海马区诱导型一氧化氮合酶表达的测定。结果:行为学实验显示,模型组大鼠学习成绩(2 min内寻找平台的时间)与记忆成绩(在2 min内跨越平台及其他3个象限相应平台位置次数)下降;各给药组大鼠的学习成绩与记忆成绩均有提高(P<0.05或P<0.01)。病理形态学显示,与假手术组相比,模型组海马区诱导型一氧化氮合酶(iNOS)表达明显增高,复智胶囊组及安理申组均可抑制iNOS表达水平增加,复智胶囊组抑制作用更为明显。结论:复智胶囊对血管性痴呆大鼠有明显改善作用。其作用机制之一可能与通过抑制iNOS活性的反应性增强来实现的。

Objective：To investigate the influence of Fuzhi Capsule on rat with vascular dementia and on expression of inducible nitric oxide synthase（iNOS） in brain hippocampus,and to explore the related mechanisms.Method： Sixty SPF rats were randomly divided into 4 groups（n = 15 each）,the vascular dementia of rat was reproduced by bilateral carotid artery ligation.Afterwards,the animals were received different treatments： Fuzhi capsule group（3.0 g.kg-1 ig daily）,aricept group（0.05 mg.g-1 ig daily）,sham operation group and model group（saline 10 mL.kg-1 ig daily）.After 4 weeks of treatments,the behavioral experiments and expression of iNOS in hippocampus were determined.Result： The experiment showed that the learning achievement（finding the platform within 2 min） and memory performance（across the platform within 2 min in the other three quadrants） in the model group were decreased.On the other hand,the learning achievement and memory performance were improved in the treatment groups（P 0.05 or P 0.01）.Pathological morphology suggested that the hippocampus iNOS expressionwas significantly increased the model group compared with sham operation group,and Fuzhi Capsule and aricept could make the expression of iNOS down.The effect of Fuzhi Capsule was more apparent compared with aricept.Conclusion： Fuzhi Capsule has a significant protective effect on vascular dementia,and the mechanism might be related with inhibiting iNOS expression.