高效液相色谱法分析组合化学合成结构相似小分子多肽

High Performance Liquid Chromatographic Analysis of StructurallySimilar Peptides by Combinatorial Chemistry Synthesis

目的：为组合化学合成结构相似小分子多肽提供快速准确的ＨＰＬＣ分析方法。方法：ＲｅｓｏｌｖＣ１８柱（５μｍ，４６ｍｍ×２００ｍｍ）。流动相：乙腈－水（２０∶８０），内含０１％三氟乙酸为流动相酸性调节剂。检测波长：２１４ｎｍ。结果：目标多肽纯度范围２６３６％～７７０２％，迁移时间２８９～３０１５ｍｉｎ。７号多肽纯度和迁移时间的ＲＳＤ分别为１９％和１４％。结论：应用本方法可快速准确分离分析结构相似的小分子多肽，尤其是组合化学合成的多肽。

Objective: To develop a HPLC method for assaying structurally similar peptides synthesized by combinatorial chemistry. Method : With Resolv C18 column(5 m,46 mm200 mm). Acetonitrile-H2O(2080) as mobile phase, 01% trifluoroacetic acid used as acidic modifier. Detector at 214 nm. Results: The purity of peptides ranges from 2636% to 7702%, migration time 2893015 min. The RSD of purity and migration time of sample 7 is 19% and 14% respectively. Conclusion:This method provides a simple and feasible way to analyze structurally similar peptides especially those synthesized by combinatorial chemistry.