唾液尿液乙肝表面抗原实验分析

The analyze of HBsAg in University HBV carriers' saliva and urine

目的实验分析唾液、尿液乙肝病毒表面抗原标志物。方法取乙肝病毒标志物阳性的健康携带者40人份唾液及40人份尿液。运用酶联免疫方法及胶体金方法检测唾液和尿液中乙肝表面抗原(HBsAg)。结果胶体金法:11人份唾液标本HBsAg呈阳性;12人份尿液标本HBsAg呈阳性。酶联免疫方法:34人份尿液标本HBsAg呈阳性。与原40人份血液乙肝HBsAg呈阳性对比,经卡方检验P<0.01。结论唾液、尿液也存在乙肝病毒表面抗原标志物。

Objective To analyze HBsAg markers in blood,urine and saliva.Methods 40 urine samples and 40 saliva samples were obtained from University HBV carriers who were dignosed to carry the HBsAg makers.Colloidal gold technique and ELISA were used to detect HBsAg markers in urine and saliva.Result Tested by colloidal gold technique HBsAg was found to be positive in 11 saliva samples and HBsAg was positive in 12 urine samples tested by ELISA.HBsAg was positive in 34 urine samples.Compared to the results of the 40 blood samples of HBsAg detection,there was significant identity by using X2 analysis P0.01.Conclusion The HBsAg makers in saliva can be used in clinical.