乳腺癌手术标本BRCA-1蛋白表达与HER-2基因扩增的临床意义

目的:研究乳腺癌患者乳腺癌手术标本中乳腺癌易感基因1(breast cancer susceptibility gene1,BRCA-1)蛋白表达、原癌基因HER-2的基因扩增与蛋白表达及其与临床病理特征之间的关系。方法:采用免疫组织化学和显色原位杂交的方法,检测乳腺癌手术切除肿瘤组织中的HER-2和BRCA-1蛋白表达以及HER-2基因扩增情况,对这两个基因的表达与临床相关病理特征之间的关系进行分析。结果:BRCA-1和HER-2蛋白表达在不同组织类型中差异无统计学意义,但在不同肿瘤大小、不同组织分级和有无淋巴结转移中差异有显著性(P<0.05)。BRCA-1蛋白表达与HER-2基因扩增率呈负相关。结论:BRCA-1和HER-2蛋白表达对乳腺癌的发生发展具有一定作用,检测BRCA-1和HER-2蛋白表达在乳腺癌的早期诊断、判断生物学行为、预测预后中有重要意义。另外BRCA-1与HER-2可能存在相关性,须进一步研究。

Objective To study the relationship of clinic pathological features and protein expression of breast cancer susceptibility gene 1 （ BRCA - 1 ） and gene amplification and protein expression of HER - 2 from surgical specimens of patients with breast cancer. Methods Detect pro- tein expression and gene amplification of HER - 2 in tumor tissue from breast cancer surgery with Immunohistochemistry and Chromogenic in situ hybridization, and analysis the relationship between the gene expression of HER - 2 and BRCA - 1 and Clinical pathology features. Results The protein expression of BRCA - 1 and HER - 2 in the different tumor Histology type groups showed no significant difference, but in different tumor si- zes, histological grade and lymph node metastasis was significantly different （ P 〈 0.05）. And protein expression of BRCA - 1 and gene amplifica- tion of HER -2 were negatively correlated. Conclusion The protein expression of BRCA - 1 and HER -2 enhanced development of breast cancer, they had a critical role in the early diagnosis, determine biological behavior and Prognosis of breast cancer. In addition, BRCA - 1 and HER -2 may be related, which requires further study.