丹酚酸A分子印迹聚合物制备及识别性能研究

Preparation of molecularly imprinted polymer and its recognition property for salvianolic acid A

以丹酚酸A为模板分子,丙烯酰胺(AM)、α-甲基丙烯酸(MAA)、2-乙烯基吡啶(2-VP)和4-乙烯基吡啶(4-VP)为功能单体,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,丙酮、乙酸乙酯、乙腈和甲醇为致孔剂,采用本体聚合法制备了一系列丹酚酸A分子印迹聚合物。通过静态平衡吸附实验和选择性实验考察了印迹聚合物的吸附性能和选择性,采用Scatchard模型研究了印迹聚合物的结合特性。结果表明,在所合成的印迹聚合物中,以丙酮为致孔剂,AM为功能单体,功能单体与模板分子摩尔比为6∶1时制备的印迹聚合物吸附量最高。通过Scatchard分析,表明该印迹聚合物中形成了两类不同的结合位点。实验结果显示,该印迹聚合物对丹酚酸A呈现出高的吸附能力和高的选择性。

A series of molecular imprinted polymers（MIP） were prepared through bulk polymerization by using Salvianolic acid A as the template molecule,acrylamide（AM）,methacrylic acid（MAA）,2-vinylpyridine（2-VP） or 4-vinylpyridine（4-VP） as the functional monomer,and ethylene glycol dimethacrylate（EGDMA） as the cross-linker in the porogen of acetone,ethyl acetate,acetonitrile or methanol.The adsorption ability and selectivity of the polymer were investigated by static equilibrium adsorption experiment and selectivity experiment,respectively.Scatchard analysis was employed to study the binding characteristics of MIP.The results indicated that the imprinted polymer prepared using acetone as the porogen,AM as the functional monomer,and the molar ratio of functional monomer to template at 6：1,exhibited the highest binding amount.Scatchard analysis revealed that two classes of binding sites were formed in the MIP.and the MIP exhibited good adsorption ability and selective ability toward Salvianolic acid A.