雌激素对去势SD雌性大鼠骨折愈合早期骨痂形成影响的实验研究

Experimental Study on the Effects of Estrogens on Callus Formation in Early Fracture Healing Stage in Ovariectomized SD Rats

目的：初步探讨雌激素在雌性ＳＤ大鼠骨折愈合早期的作用及其机制。方法：将ＳＤ大鼠分为Ａ、Ｂ、Ｃ３组，后２组摘除卵巢，２１ｄ后定支点折骨造成动物左胫腓骨闭合骨折，Ｃ组应用雌激素，分别于骨折后１、２、３、５周采取骨痂标本行ＡＫＰ测定，Ｘ线及组织学检查。结果：骨痂形成早期Ｂ组软骨骨痂较另两组稍多，但各组骨痂成熟程度无明显差异，骨痂形成后期Ｂ组骨痂结构疏松，而Ｃ组小梁致密，有编织、板层化趋势。结论：雌激素在ＳＤ大鼠骨痂形成早期轻度抑制软骨骨痂形成，骨痂形成后期有利于小梁骨形成、堆积及向编织骨的转化。

Objective:To study the effects of estrogens on the early stages of fracture healing in female SD rats.Methods:female SD rats were divided into three groups(A,B,C),Group B and C were ovariectomized.Closed fractures of left tibia were made with manual method after 21 days.Benzogynestryl was used in group C.AKP measurement,radiological and histological examinations were made in the first,second,third and fifth week after fracture.Results:Group B had a little more cartilage calluses than the other in the early callus formation stage.Trabecules of group C were thicker,denser than group B,while group B had the trend of callus osteoporosis.Conclusion:Estrogens inhibit cartilage callus formation slightly in the early callus stage and stimulate trabecular bone formation in the late callus stage in SD rats.