巨大芽孢杆菌产青霉素酰化酶培养基的优化

Optimization of Fermentation Culture Media for Production of Penicillin G Acylase from Bacillus Megaterium

通过正交实验考察了苯乙酸、酶水解酪蛋白和溶氧对产青霉素G酰化酶的影响,实验中苯乙酸作为诱导剂和碳源起作用,其消耗与溶氧具有交互作用,正交实验表采用L(934)。结果表明,苯乙酸含量与溶氧浓度是最主要的因素,最优发酵培养基组成为:酶水解酪蛋白含量为10 g/L,苯乙酸含量为20 g/L,MgCl2.6H2O含量为0.5 g/L,FeCl3.6H2O含量为0.002 g/L,250 mL三角瓶装液量为30 mL。

Phenyalacetic acid,tryptone and dissolved oxygen were tested for the production of penicillin G acylase using bacillus megaterium by L9（3^4） orthogonal experimental design with phenyalacetic acid as inducer and carbon source,interated with dissolved oxygen in the culture.The results showed main factors were the interaction between the concentr-ation of phenyal acetic acid and dissolved oxygen（DO）,the best media： tryptone 10 g/L,phenylacetic acid 20 g/L,MgCl2·6H2O 0.5 g/L,FeCl3·6H2O 0.002 g/L,30 mL/250 mL flask.