面神经损伤后TGF-β的表达与schwann细胞数量变化关系的研究

The Correlate of Proliferation of the Schwann Cells and Expression of Transforming Growth Factor Beta in Facial Nerve Crushed Injury

目的观察兔面神经夹伤后转化生长因子β(transforming growth factor beta,TGF-β)的表达及施万细胞(schwann cells,SCs)数量的变化,了解两者在面神经损伤修复过程中的相互关系及相互作用。方法取20只新西兰大白兔,造成面神经钳夹伤,于伤后1、3、7、14、21d取材分别进行组织学、免疫组化观察。结果 3d时神经夹伤处TGF-β抗原呈阳性反应,施万细胞增多;7d时TGF-β表达呈强阳性反应,为表达高峰,损伤处的施万细胞明显增多;14d时TGF-β表达的量有所减少,施万细胞数量亦无增多趋势;21d时施万细胞数量和TGF-β抗原表达的量均下降。结论 TGF-β参与面神经损伤的病理过程,并与施万细胞数量的变化存在一定的同步性,证实了两者之间存在相互作用。

Objective To observe the changes of transforming growth factor beta（TGF-β）expression and the rule of the schwann cells proliferation after rabbit facial nerve crushed injury and learning about the correlate of TGF-βand Schwann cells in facial nerve injury and repair.Methods Twenty rabbits were used.The facial nerves were curshed with a special clamp.The histology and immunohistochemistry examination were performed respectively after 1,3,7,14,21days injury.Results The TGF-βantibody in crushed facial nerve was positive with the Schwann cells increased after 3 days injury.The Schwann cells increased greatly and the TGF-βexpression enhanced after 7 days injury.The expression of TGF-βdecreased gradually and the Schwann cells no increasing after 14 days injury.The Schwann cells and the TGF-βexpression decreased greatly after 21 days injury.Conclusion The TGF-βparticipate in the pathoiogic process of facial nerve damage,and correlate to the proliferation of Schwann cells,Confirm the interaction between them.